Camelids nanobodies: versatile tools for molecular and structural biology studies
Dr Alain ROUSSEL
AFMB, Marseille, France
Monday, November 18th 2019 - 2 p.m.
- Auditorium, IGBMC
Hosted by Integrated structural Biology, Bruno KLAHOLZ
In recent years, the use of single-domain camelid immunoglobulins, termed vHHs or nanobodies, has seen increasing growth in biotechnology, pharmaceutical applications and structure/function research. The usefulness of nanobodies in molecular and structural biology is now firmly established whether to solve 3D structures or to perform functional studies. After describing our platform dedicated to the selection and production of nanobodies, I will present the recent results we have obtained in the structural studies of bacterial secretion systems (T2SS, T6SS and T9SS). Finally I will present our current work on the development of neutralizing nanobodies against Ebola virus.
Desmyter, A., Spinelli, S., Roussel, A., and Cambillau, C. (2015) Camelid nanobodies: killing two birds with one stone. Current opinion in structural biology 32C, 1-8
Douzi, B., Trinh, N. T. T., Michel-Souzy, S., Desmyter, A., Ball, G., Barbier, P., Kosta, A., Durand, E., Forest, K. T., Cambillau, C., Roussel, A., and Voulhoux, R. (2017) Unraveling the Self-Assembly of the Pseudomonas aeruginosa XcpQ Secretin Periplasmic Domain Provides New Molecular Insights into Type II Secretion System Secreton Architecture and Dynamics. MBio 8
Nguyen, V. S., Logger, L., Spinelli, S., Legrand, P., Huyen Pham, T. T., Nhung Trinh, T. T., Cherrak, Y., Zoued, A., Desmyter, A., Durand, E., Roussel, A., Kellenberger, C., Cascales, E., and Cambillau, C. (2017) Type VI secretion TssK baseplate protein exhibits structural similarity with phage receptor-binding proteins and evolved to bind the membrane complex. Nat Microbiol 2, 17103
Leone, P., Roche, J., Vincent, M. S., Tran, Q. H., Desmyter, A., Cascales, E., Kellenberger, C., Cambillau, C., and Roussel, A. (2018) Type IX secretion system PorM and gliding machinery GldM form arches spanning the periplasmic space. Nat Commun 9, 429
Alain Roussel, Director of Research CNRS. Deputy director of AFMB, Marseille, France. Head of the «Host-Pathogen Interaction » team (http://www.afmb.univ-mrs.fr/host-pathogen-interactions). Scientific coordinator of the IbiSA platform of Structural Biology at AFMB, which is supported by the French Infrastructure for Integrated Structural Biology (FRISBI). Member of the executive committee of FRISBI. Specialist of X-ray crystallography. Fields of interest: Innate immunity, Bacterial secretion systems, Monoclonal antibodies.