4 research departments
750 employees
45 nationalities
55 research teams
16 ERC laureates
260 publications per year
24000 m² lab area

Support us through

Fondation universite de Strasbourg

Key figures 2019

9 congresses, workshops and meetings
5 special seminars
0 internal seminars
24 thesis


Quick Links


Spatially resolved transcriptomics in human cell nuclei

Dr Jorg MORF
Babraham Institute, Cambridge, United Kingdom

Thursday, November 14th 2019 - 10 a.m. - Auditorium, IGBMC
Hosted by Functional genomics and cancer, Nacho MOLINA

Spatial transcriptomics aims to understand how the ensemble of RNA molecules in tissues and cells is organized in 3D space. To probe the spatial RNA organization, we developed Proximity RNA-seq, a ligation-free method based on massive-throughput RNA barcoding of subcellular particles and assemblies in water-in-oil emulsion droplets, followed by sequencing. In human nuclei, we identified co-localization preferences for pairs and groups of nuclear-retained and nascent RNAs and detect RNA-rich, phase-separated structures, such as the nucleolus and speckles. Unexpectedly, the transcriptional output of the genome measured by Proximity RNA-seq suggests that heterochromatin is interspersed with nucleic acid-dense regions encompassing active genes with faster transcription kinetics.

Imprimer Envoyer

Université de Strasbourg

IGBMC - CNRS UMR 7104 - Inserm U 1258
1 rue Laurent Fries / BP 10142 / 67404 Illkirch CEDEX / France Tél +33 (0)3 88 65 32 00 / Fax +33 (0)3 88 65 32 01 / directeur.igbmc@igbmc.fr