Structure and Function of the Spliceosome
Pr Rui ZHAO
University of Colorado Department of BioChem and Molecular Genetics Denver, United States
Thursday, May 28th 2020 - 11 a.m.
- Auditorium, IGBMC
Hosted by Integrated structural Biology, Marat YUSUPOV
Pre-mRNA splicing is an essential step in the gene expression pathway of all eukaryotes. The splicing reaction is catalyzed by the spliceosome, a huge RNA-protein complex made of five snRNAs and over 100 different proteins. Spliceosome undergoes dramatic compositional and conformational rearrangements through the splicing cycle, forming at least 10 distinct complexes. We have recently determined the cryo-EM structures of U1 snRNP as well as the spliceosomal E and P complexes. I will discuss these structures and the insight they provide in the mechanism of intron definition, exon definition, back-splicing, as well as exon ligation and release.