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workshop

Instruct-FRISBI course: Preparation & characterization of macromolecular complexes

lundi 19 novembre - vendredi 23 novembre 2018 - Salle de conférence E1031, CBI
Organisé(e) par Catherine BIRCK & Arnaud POTERSZMAN

Co-organizer: Jean-Paul Concordet, MNHN, Paris FR

 

No registration fees

Place limited to 16 participants

 

This Instruct-FRISBI practical course will provide participants with an overview of strategies for preparation and biophysical characterization of macromolecular complexes in view of biochemical and structural studies. The emphasis of the workshop will be placed on purification of macromolecular assemblies from endogenous sources using engineered cell lines and in reconstitution of multiprotein complexes using the baculovirus expression system.

The course is intended primarily for post?doctoral researchers, PhD students and researchers in structural biology. Selected participants will improve their theoretical background and gain experience in designing experimental strategies relevant for their projects.

 

1/ Engineered cell lines using the CRISPR/Cas9 genome editing technology. Strategies to generate human knock-in stable cell lines for isolation of difficult-to-express proteins and high resolution imaging.

 

2/ Production of recombinant multiprotein complexes using the baculovirus expression system. Construct and primer design, experimental setup and basic insect cell manipulations.

 

3/ Biophysical characterization and sample optimization using complementary methods including nanoDSF, AUC-SV, SEC-MALS and EM analysis of negatively stained particles.

 

Site de l'événement

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Université de Strasbourg
INSERM
CNRS

IGBMC - CNRS UMR 7104 - Inserm U 1258
1 rue Laurent Fries / BP 10142 / 67404 Illkirch CEDEX / France Tél +33 (0)3 88 65 32 00 / Fax +33 (0)3 88 65 32 01 / directeur.igbmc@igbmc.fr