Reference : Post doc B.R-S-M
Postdoctoral position in the field of Molecular Immunology.
The Reina lab is seeking a highly motivated and experienced postdoctoral fellow to study the molecular mechanisms driving B cell receptor diversification during immune responses, with a particular focus on immunoglobulin class switch recombination (CSR). CSR modulates antibody effector functions by replacing the isotype expressed (from IgM to IgG, IgA or IgE) through a DNA recombination reaction (occurring at the IgH locus) that requires double stranded DNA break (DSBs) intermediates induced by activation-induced cytidine deaminase (AID). These DSBs activate DNA damage response proteins to promote appropriate repair and long-range recombination. While on-target lesions are crucial for Ig diversification, off-target lesions contribute to malignant cell transformation and AID has been strongly implicated in the initiation of cancer. Despite the significant potential of AID to inflict collateral DNA damage, most B cells expressing AID do not suffer widespread mutation or chromosome instability. Therefore, it appears that specific regulatory mechanisms actively restrict AID's oncogenic potential. The selected postdoctoral fellow will build on the expertise of the lab, in particular in genome editing using the CRISPR/Cas9 system, to investigate the molecular mechanisms that control the function of AID during CSR and that limit its oncogenic potential.
Applicants must be highly motivated and have a PhD or equivalent doctoral degree with at least 3 years of proven research experience in Molecular Biology, Immunology or Biochemistry. Candidates should have a good publication record and should be fluent in English (French is not required). Good communication skills (oral and written) and the ability to work in a team are essential.
The Reina Lab has a strong publication record and has made in the past few years substantial contributions to the understanding of the molecular mechanisms that mediate CSR. Approaches used include molecular and cellular techniques, genome editing (CRISPR/Cas9), gene targeting, mouse genetics, mass spectrometry, in vitro cell differentiation assays, flow cytometry, ChIP-Seq and 4C-Seq analysis. The candidate will benefit from a rich scientific environment at IGBMC, including access to several state-of–the art research facilities.
Applications should be addressed by e-mail to Dr. Bernardo Reina-San-Martin (firstname.lastname@example.org) and include a complete curriculum vitae with a short statement of of past and future research interests and the contact information (phone number and email) of at least two referees.