4 research departments
750 employees
45 nationalities
55 research teams
16 ERC laureates
260 publications per year
24000 m² lab area

Support us through

Fondation universite de Strasbourg

photo

Head

Jocelyn LAPORTE
jocelyn.laporte@igbmc.fr
Tel. : +33 (0)3 88 65 34 12

Key figures

52 research teams
127 researchers
34 post-doctoral fellows
31 PhD students
109 engineers & technicians
110 administrators & general services 110 ICS employees

Quick Links

Job opportunities

Identification And Functional Characterization Of Novel Genes For Myopathies

Reference : Equipe Jocelyn Laporte

Offer publication : June 23, 2021

Skeletal muscle represent half of the dried body weight and is key for many physiological functions including metabolism and movement. A plethora of muscle diseases are known, including severe congenital myopathies associated to life-threatening muscle weakness. Main bottlenecks are that half of patients lack a genetic diagnosis, the pathological mechanisms are not understood, and there is no specific cure for most of them. Thus we aim to identify novel myopathy genes that will represent novel therapeutic targets.

To this goal, the PhD student will use next generation sequencing strategies, exome and genome sequencing of patients DNA, and RNAseq/transcriptome from patient muscles, and process these big datasets through bioinformatics pipelines to identify mutated genes in patients with myopathies.

The functional impact of mutations will be confirmed by in vitro assays with recombinant proteins and transfected and differentiated muscle cells followed by confocal and video microscopies. The importance of these new myopathy genes for muscle function will be evaluated in vivo in an animal model (zebrafish or mice depending on the gene conservation) with CRISPR/Cas9 genome editing, RNA interference and adeno-associated virus. Animals will be phenotyped with simple motor tests, histology, electron microscopy and confocal imaging. This will also provide faithful laboratory models in which therapies may be later tested.

The success of this project will lead to a better diagnosis and patient care, and the identified genes will increase our understanding of the pathomechanisms and represent novel therapeutic targets

Your application

Imprimer Envoyer

Université de Strasbourg
INSERM
CNRS

IGBMC - CNRS UMR 7104 - Inserm U 1258
1 rue Laurent Fries / BP 10142 / 67404 Illkirch CEDEX / France Tél +33 (0)3 88 65 32 00 / Fax +33 (0)3 88 65 32 01 / directeur.igbmc@igbmc.fr