IGBMC : Institut de la Génétique et de la Biologie Moléculaire et Cellulaire

• Researchers

  Norbert GHYSELINCK

  Manuel MARK

• Post-Doctoral Fellowships

  Aurore GELY PERNOT

• Phd Students

  Mathilde RAVERDEAU

• Engineers & Technicians

  Christine DENNEFELD

  Betty FERET

  Muriel KLOPFENSTEIN

• Master Students

  Mouni STAMBOULI

Development & stems cells

Retinoic acid signaling pathways driving stem spermatogonia ontogenesis and differentiation

Retinoic acid (RA), the biologically active metabolite of vitamin A, exerts a wide range of cellular effects by regulating proliferation, differentiation and apoptosis. At the cellular level, the action of RA is controlled through the modulated expression of cellular enzymes producing or degrading RA, retinaldehyde dehydrogenases (RALDH) or cytochrome P450 hydroxylases (CYP26), respectively. Then, acting in an hormone-like manner, RA binds to nuclear receptors (RAR) that function as transcriptional regulators through controlling, usually in the form of heterodimers with rexinoid receptors (RXR), the expression of RA target genes. Among the various tissues whose physiology depends upon vitamin A action, the seminiferous epithelium of the testis represents the most remarkable paradigm to investigate the pleiotropic effects of RA in vivo, as it integrates the problematic of stem cell renewal, cell proliferation, switching from mitotic to meiotic cell division, programmed cell death and paracrine signaling. Using a combination of innovative genetic, pharmacological and molecular approaches in the mouse, we are studying the cellular and molecular mechanisms that underlie the capabilities of RA to promote spermatogonia differentiation and beyond the differentiation of normal stem cells in vivo.

• Current projects

• Collaborations

• Prizes/Awards

• News

  • April 29, 2011 - Identification of new tumor suppressors in hepatocellular carcinoma : the TRIM proteins

• Publications

  • Spermatogonia differentiation requires retinoic acid receptor .

    Gely-Pernot A, Raverdeau M, Célébi C, Dennefeld C, Feret B, Klopfenstein M, Yoshida S, Ghyselinck NB, Mark M.

    Endocrinology Jan 2012 ; 153:438-49 .

  • Genetic disruption of aurora B uncovers an essential role for aurora C during early mammalian development

    Fernandez-Miranda G., Trakala M., Martin J., Escobar B., Gonzalez A., Ghyselinck N.B., Ortega S., Canamero M., de Castro I.P., Malumbres M.

    Development Jul 2011 ; 138:2661-72 .

  • RA-RAR-beta counteracts myelin-dependent inhibition of neurite outgrowth via Lingo-1 repression

    Puttagunta R., Schmandke A., Floriddia E., Gaub P., Fomin N., Ghyselinck N.B., Di Giovanni S.

    J Cell Biol June 27, 2011 ; 193:1147-56 .

  • Retinoids enhance glucocorticoid-induced apoptosis of T cells by facilitating glucocorticoid receptor-mediated transcription

    Toth K., Sarang Z., Scholtz B., Brazda P., Ghyselinck N., Chambon P., Fesus L., Szondy Z.

    Cell Death Differ May 2011 ; 18:783-92 .

  • Standardized Post-Mortem Examination and Fixation Procedures for Mutant and Treated Mice

    Antal M.C., muller S., Wendling O, Herault Y, Mark M.

    Current Protocols in Mouse Biology 2011 ; 1:1-38 .

  • TIF1beta association with HP1 is essential for post-gastrulation development, but not for Sertoli cell functions during spermatogenesis

    Herzog M., Wendling O., Guillou F., Chambon P., Mark M., Losson R., Cammas F.

    Dev Biol Feb. 15, 2011 ; 350:548-58 .

  • Transcription cofactors TRIM24, TRIM28, and TRIM33 associate to form regulatory complexes that suppress murine hepatocellular carcinoma

    Herquel B., Ouararhni K., Khetchoumian K., Ignat M., Teletin M., Mark M., Bechade G., Van Dorsselaer A., Sanglier-Cianferani S., Hamiche A., Cammas F., Davidson I., Losson R.

    Proc Natl Acad Sci U S A May 17, 2011 ; 108:8212-7 .

  • Retinoic acid drives aryl hydrocarbon receptor expression and is instrumental to dioxin-induced toxicity during palate development.

    Jacobs H, Dennefeld C, Féret B, Viluksela M, Håkansson H, Mark M, Ghyselinck NB.

    Environ Health Perspect Nov 2011 ; 119:1590-5 .

  • Negative control of Smad activity by ectodermin/Tif1gamma patterns the mammalian embryo

    Morsut L., Yan K.P., Enzo E., Aragona M., Soligo S.M., Wendling O., Mark M., Khetchoumian K., Bressan G., Chambon P., Dupont S., Losson R., Piccolo S.

    Development Aug. 1, 2010 ; 137:2571-8 .

  • Retinoic acid receptor (RAR)-alpha is not critically required for mediating retinoic acid effects in the developing mouse retina

    Cammas L., Trensz F., Jellali A., Ghyselinck N.B., Roux M.J., Dolle P.

    Invest Ophthalmol Vis Sci Jun 2010 ; 51:3281-90 .

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