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TARDIS: a universal method at everyone hands to identify rare RNAs

Schematic overview of TARDIS and its modular workflows.

TARDIS, a targeted RNA directional sequencing method for rare RNA discovery.

Portal MM(1), Pavet V(1), Erb C(1), Gronemeyer H(1).

Nat Protoc Dec 2015

Oct. 29, 2015

During the last years, RNA sequencing has become a widely used tool to study RNA expression. However, a cost-effective and universal protocol capable of dealing with any source of input RNA was not available till date. In a study coordinated by Maximiliano Portal and Hinrich Gronemeyer at the IGBMC, the scientists have developed a method for RNA-sequencing library preparation that is capable of querying the entire transcriptome of any organism, regardless of RNA length, function, the presence or absence of poly-A or biogenesis mechanism. Called TARDIS (for Targeted Directional Sequencing RNA), this protocol was published on October 29th in the journal Nature Protocols.

Importantly, TARDIS presents two major advantages over current strategies, as it enables the identification of RNAs that escape detection by existing technologies and it also empowers the detection of rare or low-expressed RNAs. Its potential has already been demonstrated in a recent study in which TARDIS was used to analyze the transcriptome of a «silent» region (considered as transcriptionally inactive) allowing the discovery of a novel family of non-coding RNAs present in human cells.

Notably, TARDIS is easy to implement, as the required reagents are generally available in molecular biology labs, thus substantially reducing cost linked to RNA-seq library preparation. Built over a modular design, TARDIS can be in principle applied for different purposes under any experimental context of interest in a highly reproducible manner, thus representing an undeniable cost-effective technology for RNA discovery.

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