IGBMC : Institut de la Génétique et de la Biologie Moléculaire et Cellulaire

• Researchers

  Izabela SUMARA

• Post-Doctoral Fellows

  Arantxa AGOTE ARAN

  Paulo Jorge GOMES PEREIRA DA COSTA

  Evanthia PANGOU

• PhD Students

  Lucile GUERBER

  Katerina JERABKOVA

  Yongrong LIAO

• Engineers & Technicians

  Charlotte KLEISS

Development and stem cells

Cell cycle and ubiquitin signaling

Cell division is instrumental for all living organisms and defects in its regulatory systems are linked to cellular transformation. Cell division requires duplication of all genetic material during S-phase followed by its precise partitioning between two daughter cells during mitosis. The cell cycle machinery involves many critical factors and enzymes that need to be precisely regulated in time and space. Thus, both their timely expression and correct localization to the subcellular compartments must be achieved during different morphological transitions.
Research in our group focuses on the role of the ubiquitin conjugation system in spatio-temporal control of cell division. We are using advanced microscopy and proteomic approaches to identify and characterize the key components of the ubiquitin-conjugation machinery in human cells (E3-ligases, Deubiquitinating enzymes, Ubiquitin-binding proteins) as well as the critical ubiquitination substrates that play a role during cell cycle progression. Our team is particularly interested in understanding the mechanisms coordinating the mitotic events with completion of cytokinesis. Studying the basic molecular mechanisms of cell division will hopefully help us to understand the origins of genomic instability and thereby cellular transformation.

• Current projects

  Our research group will focus on the major question: What are the molecular mechanisms of cell cycle and genome stability?
  Specifically:
  1. What are the molecular mechanisms of faithful cell cycle and how do Cul3-based E3-ligases regulate cell   division?
  2. What are the novel ubiquitination substrates that are required for faithful cell division?
  3. What are the novel components of the ubiquitin system, which are required for mitosis in mammalian
  cells?
  4. What is the role of Cul3 and UPS during cancer development?

• Collaborations

  - Daniel Gerlich, Institute of Biochemistry ,ETH Zurich, Switzerland
  - Jason Swedlow, Wellcome Trust Biocentre School of Life, Sciences University of Dundee, Scotland
  - Laurent Brino, IGBMC, France
  - Christophe Romier, IGBMC, France
  - Olivier Poch, IGBMC, France

• Prizes/Awards

  • Izabela Sumara - Sanofi Innovation Awards Program Europe (iAwards) - Sanofi - 2018
  • Izabela Sumara - Fellow of the University of Strasbourg Institute for Advanced Study - USIAS - 2014
  • Izabella SUMARA - ATIP - AVENIR starting grant - Inserm / CNRS - 2011

• News

  • Jan. 11, 2016 - Reading out the ubiquitin code during mitosis
  • March 3, 2013 - A new pathway regulating mitosis

• Publications

  • Intraflagellar Transport Complex B Proteins Regulate the Hippo Effector Yap1 during Cardiogenesis

    Marina Peralta 1 , Laia Ortiz Lopez 1 , Katerina Jerabkova 1 , Tommaso Lucchesi 2 , Benjamin Vitre 3 , Dong Han 4 , Laurent Guillemot 4 , Chaitanya Dingare 5 , Izabela Sumara 1 , Nadia Mercader 6 , Virginie Lecaudey 5 , Benedicte Delaval 3 , Sigolène M Meilhac 4 , Julien Vermot 7

    Cell Press 2020 July 21 2020 ; Vol 32: .

  • Spatial control of nucleoporin condensation by fragile X-related proteins

    Arantxa Agote-Aran # 1 2 3 4 , Stephane Schmucker # 1 2 3 4 , Katerina Jerabkova 1 2 3 4 , Inès Jmel Boyer 1 2 3 4 , Alessandro Berto 5 6 , Laura Pacini 7 , Paolo Ronchi 8 , Charlotte Kleiss 1 2 3 4 , Laurent Guerard 9 , Yannick Schwab 8 10 , Hervé Moine 1 2 3 4 , Jean-Louis Mandel 1 2 3 4 , Sebastien Jacquemont 11 12 , Claudia Bagni 7 13 , Izabela Sumara 1 2 3 4

    The EMBO Journal 2020 July 24 2020 ; : .

  • Deubiquitylase UCHL3 regulates bi-orientation and segregation of chromosomes during mitosis

    Katerina Jerabkova 1 2 3 4 5 6 , Yongrong Liao 1 2 3 4 , Charlotte Kleiss 1 2 3 4 , Sadek Fournane 1 2 3 4 , Matej Durik 1 2 3 4 , Arantxa Agote-Arán 1 2 3 4 , Laurent Brino 1 2 3 4 , Radislav Sedlacek 5 6 7 , Izabela Sumara 1 2 3 4

    The Faseb Journal 2020 Aug 1 2020 ; : .

  • Cullin 3, a cellular scripter of the non-proteolytic ubiquitin code.

    Jerabkova K(1), Sumara I(2).

    Semin Cell Dev Biol Dec. 23, 2018 .

  • Recapitulating early development of mouse musculoskeletal precursors of the paraxial mesoderm in vitro.

    Chal J(1)(2)(3)(4), Al Tanoury Z(1)(2)(3)(4), Oginuma M(1)(2)(3)(4), Moncuquet P(1), Gobert B(1)(5), Miyanari A(1), Tassy O(1), Guevara G(2)(3)(4), Hubaud A(1)(2)(3)(4), Bera A(1), Sumara O(1), Garnier JM(1), Kennedy L(1), Knockaert M(1)(2)(3)(4), Gayraud-Morel B(6)(7), Tajbakhsh S(6)(7), Pourquie O(8)(2)(3)(4).

    Development March 19, 2018 ; 145:dev157339 .

  • The MTM1-UBQLN2-HSP complex mediates degradation of misfolded intermediate filaments in skeletal muscle.

    Gavriilidis C(1)(2)(3)(4), Laredj L(1)(2)(3)(4), Solinhac R(5), Messaddeq N(2)(3)(4), Viaud J(5), Laporte J(1)(2)(3)(4), Sumara I(2)(3)(4)(6), Hnia K(7)(8)(9)(10)(11).

    Nat Cell Biol Feb 2018 ; 20:198-210 .

  • UBASH3B-mediated silencing of the mitotic checkpoint: Therapeutic perspectives in cancer.

    Krupina K(1)(2), Kleiss C(1), Awal S(1), Rodriguez-Hernandez I(3), Sanz-Moreno V(3), Sumara I(1).

    Mol Cell Oncol Nov. 30, 2017 ; 5:e1271494 .

  • Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.

    Courtheoux T(1), Enchev RI(1), Lampert F(1), Gerez J(1), Beck J(1), Picotti P(1), Sumara I(2), Peter M(1).

    Nat Commun Sept. 19, 2016 ; 7:12810 .

  • Corrigendum: Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.

    Courtheoux T, Enchev RI, Lampert F, Gerez J, Beck J, Picotti P, Sumara I, Peter M.

    Nat Commun Oct. 31, 2016 ; 7:13487 .

  • Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3.

    Magliarelli HF(1), Matondo M(2), Mészáros G(1,)(3), Goginashvili A(1), Erbs E(1), Zhang Z(1), Mihlan M(1), Wolfrum C(4,)(5), Aebersold R(2,)(5), Sumara I(1), Ricci R(1,)(3).

    Cell Death Dis Oct. 13, 2016 ; 7:e2411 .

  See all publications=>

• Job opportunities

• Alumni

• Videos

• Thesis subjects

  • Linking Ubiquitin Signaling To Fragile X Syndrome (Fxs) (H/F)
  • Targeting Mitotic Ubiquitin Receptor Protein Ubash3b For Cancer Therapies (H/F)