IGBMC : Institut de la Génétique et de la Biologie Moléculaire et Cellulaire

• Researchers

  Izabela SUMARA

• Post-Doctoral Fellows

  Paulo Jorge GOMES PEREIRA DA COSTA

  Evanthia PANGOU

• PhD Students

  Arantxa AGOTE ARAN

  Lucile GUERBER

  Katerina JERABKOVA

  Yongrong LIAO

• Engineers & Technicians

  Charlotte KLEISS

Development and stem cells

Cell cycle and ubiquitin signaling

Cell division is instrumental for all living organisms and defects in its regulatory systems are linked to cellular transformation. Cell division requires duplication of all genetic material during S-phase followed by its precise partitioning between two daughter cells during mitosis. The cell cycle machinery involves many critical factors and enzymes that need to be precisely regulated in time and space. Thus, both their timely expression and correct localization to the subcellular compartments must be achieved during different morphological transitions.
Research in our group focuses on the role of the ubiquitin conjugation system in spatio-temporal control of cell division. We are using advanced microscopy and proteomic approaches to identify and characterize the key components of the ubiquitin-conjugation machinery in human cells (E3-ligases, Deubiquitinating enzymes, Ubiquitin-binding proteins) as well as the critical ubiquitination substrates that play a role during cell cycle progression. Our team is particularly interested in understanding the mechanisms coordinating the mitotic events with completion of cytokinesis. Studying the basic molecular mechanisms of cell division will hopefully help us to understand the origins of genomic instability and thereby cellular transformation.

• Current projects

  Our research group will focus on the major question: What are the molecular mechanisms of cell cycle and genome stability?
  Specifically:
  1. What are the molecular mechanisms of faithful cell cycle and how do Cul3-based E3-ligases regulate cell   division?
  2. What are the novel ubiquitination substrates that are required for faithful cell division?
  3. What are the novel components of the ubiquitin system, which are required for mitosis in mammalian
  cells?
  4. What is the role of Cul3 and UPS during cancer development?

• Collaborations

  - Daniel Gerlich, Institute of Biochemistry ,ETH Zurich, Switzerland
  - Jason Swedlow, Wellcome Trust Biocentre School of Life, Sciences University of Dundee, Scotland
  - Laurent Brino, IGBMC, France
  - Christophe Romier, IGBMC, France
  - Olivier Poch, IGBMC, France

• Prizes/Awards

  • Izabela Sumara - Sanofi Innovation Awards Program Europe (iAwards) - Sanofi - 2018
  • Izabela Sumara - Fellow of the University of Strasbourg Institute for Advanced Study - USIAS - 2014
  • Izabella SUMARA - ATIP - AVENIR starting grant - Inserm / CNRS - 2011

• News

  • Jan. 11, 2016 - Reading out the ubiquitin code during mitosis
  • March 3, 2013 - A new pathway regulating mitosis

• Publications

  • Cullin 3, a cellular scripter of the non-proteolytic ubiquitin code.

    Jerabkova K(1), Sumara I(2).

    Semin Cell Dev Biol Dec. 23, 2018 .

  • Recapitulating early development of mouse musculoskeletal precursors of the paraxial mesoderm in vitro.

    Chal J(1)(2)(3)(4), Al Tanoury Z(1)(2)(3)(4), Oginuma M(1)(2)(3)(4), Moncuquet P(1), Gobert B(1)(5), Miyanari A(1), Tassy O(1), Guevara G(2)(3)(4), Hubaud A(1)(2)(3)(4), Bera A(1), Sumara O(1), Garnier JM(1), Kennedy L(1), Knockaert M(1)(2)(3)(4), Gayraud-Morel B(6)(7), Tajbakhsh S(6)(7), Pourquie O(8)(2)(3)(4).

    Development March 19, 2018 ; 145:dev157339 .

  • The MTM1-UBQLN2-HSP complex mediates degradation of misfolded intermediate filaments in skeletal muscle.

    Gavriilidis C(1)(2)(3)(4), Laredj L(1)(2)(3)(4), Solinhac R(5), Messaddeq N(2)(3)(4), Viaud J(5), Laporte J(1)(2)(3)(4), Sumara I(2)(3)(4)(6), Hnia K(7)(8)(9)(10)(11).

    Nat Cell Biol Feb 2018 ; 20:198-210 .

  • UBASH3B-mediated silencing of the mitotic checkpoint: Therapeutic perspectives in cancer.

    Krupina K(1)(2), Kleiss C(1), Awal S(1), Rodriguez-Hernandez I(3), Sanz-Moreno V(3), Sumara I(1).

    Mol Cell Oncol Nov. 30, 2017 ; 5:e1271494 .

  • Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.

    Courtheoux T(1), Enchev RI(1), Lampert F(1), Gerez J(1), Beck J(1), Picotti P(1), Sumara I(2), Peter M(1).

    Nat Commun Sept. 19, 2016 ; 7:12810 .

  • Corrigendum: Cortical dynamics during cell motility are regulated by CRL3(KLHL21) E3 ubiquitin ligase.

    Courtheoux T, Enchev RI, Lampert F, Gerez J, Beck J, Picotti P, Sumara I, Peter M.

    Nat Commun Oct. 31, 2016 ; 7:13487 .

  • Liver ubiquitome uncovers nutrient-stress-mediated trafficking and secretion of complement C3.

    Magliarelli HF(1), Matondo M(2), Mészáros G(1,)(3), Goginashvili A(1), Erbs E(1), Zhang Z(1), Mihlan M(1), Wolfrum C(4,)(5), Aebersold R(2,)(5), Sumara I(1), Ricci R(1,)(3).

    Cell Death Dis Oct. 13, 2016 ; 7:e2411 .

  • Mutations in the HECT domain of NEDD4L lead to AKT-mTOR pathway deregulation and cause periventricular nodular heterotopia.

    Broix L(1,)(2,)(3,)(4,)(5), Jagline H(1,)(2,)(3,)(4), L Ivanova E(1,)(2,)(3,)(4), Schmucker S(1,)(2,)(3,)(4), Drouot N(1,)(2,)(3,)(4), Clayton-Smith J(6), Pagnamenta AT(7), Metcalfe KA(6), Isidor B(8), Louvier UW(9), Poduri A(10), Taylor JC(7), Tilly P(1,)(2,)(3,)(4), Poirier K(5), Saillour Y(5), Lebrun N(5), Stemmelen T(1,)(2,)(3,)(4), Rudolf G(1,)(2,)(3,)(4), Muraca G(5), Saintpierre B(5), Elmorjani A(5); Deciphering Developmental Disorders study, Moïse M(11), Weirauch NB(12), Guerrini R(13), Boland A(14), Olaso R(14), Masson C(15), Tripathy R(16), Keays D(16), Beldjord C(17), Nguyen L(11), Godin J(1,)(2,)(3,)(4), Kini U(18), Nischké P(15), Deleuze JF(14), Bahi-Buisson N(19), Sumara I(1,)(2,)(3,)(4), Hinckelmann MV(1,)(2,)(3,)(4), Chelly J(1,)(2,)(3,)(4,)(20).

    Nat Genet nov 2016 2016 ; 48:1349-1358 .

  • Ubiquitin Receptor Protein UBASH3B Drives Aurora B Recruitment to Mitotic Microtubules.

    Krupina K(1), Kleiss C(1), Metzger T(1), Fournane S(1), Schmucker S(1), Hofmann K(2), Fischer B(1), Paul N(3), Porter IM(4), Raffelsberger W(5), Poch O(6), Swedlow JR(4), Brino L(1), Sumara I(7).

    Dev Cell Jan. 11, 2016 ; 36:63-78 .

  • Insulin granules. Insulin secretory granules control autophagy in pancreatic beta cells.

    Goginashvili A, Zhang Z, Erbs E, Spiegelhalter C, Kessler P, Mihlan M, Pasquier A, Krupina K, Schieber N, Cinque L, Morvan J, Sumara I, Schwab Y, Settembre C, Ricci R.

    Science Feb. 20, 2015 ; 347:878-82 .

  See all publications=>

• Job opportunities

• Alumni

• Videos

• Thesis subjects

  • Targeting Mitotic Ubiquitin Receptor Protein Ubash3b For Cancer Therapies (H/F)
  • Linking Ubiquitin Signaling To Fragile X Syndrome (Fxs) (H/F)