The CBI welcomes a new instrument: Fidabio
Centre for integrative biology officially welcomes a new instrument: Fidabio. This will enable the behaviour of biomolecules to be characterised by monitoring changes in the hydrodynamic radius (Rh). With a wide range of applications, from structural studies (interactions between biomolecules, structural changes, etc.) to affinity measurements (Kd) in complex media, this rapid and inexpensive technique can be used for many research projects.
A simple, fast and inexpensive method
The Fidabio tool allows the direct measurement of biomolecule size in solution by determining the hydrodynamic radii (or diffusion coefficients) using Taylor dispersion analysis (TDA). This is a simple, fast and absolute method, which consumes only a few nL of sample. It consists of injecting a fluorescent marker (a biomolecule that has been previously labelled with a fluorescent element) into an open capillary tube and mobilising it under the influence of a hydrodynamic flow containing the sample.
This technique is capable of detecting size changes up to 5% variation in hydrodynamic radius. It is therefore a particularly sensitive technique. With short handling times, between 3 and 5 minutes per pre-prepared sample, this technique allows reliable results to be obtained with a small number of repetitions.
A technique that can be adapted to various situations
The areas of application of the Fidabio technique are as follows:
The possibilities are wide and can be easily adapted to your research projects. Note that this technique also works in cases where the protein studied is located in a "complex medium", such as measuring the presence of antibodies in the plasma of an individual.
Getting access to the Fidabio instrument
The tool is already in place at the IGBMC. As the reference centre in France for this technique, this gives the CBI a privileged link with the company Fidabio. Do not hesitate to contact Marc Ruff to assist you in your projects, particularly in the use of the tool or in the choice of the indicator in relation to the analyte you intend to study.
To give you an idea of the possibilities opened up by this solution, you can, for example, consult this article in which the researchers used this technique: read the article.