Structural biology of epigenetic targets
Structural biology of epigenetic targets
Chromatin structure is a major barrier to all nuclear processes and its modulation is essential for correct cell growth. There is now clear evidence that defects in chromatin modulation are responsible for a wide range of diseases, including cancer. Epigenetic modifications have a large impact on chromatin structure, acting in synergy with ATP-dependent remodelers, histone variants and histone chaperones to regulate nuclear mechanisms. By combining state-of-the-art biochemical and crystallographic techniques, we aim to understand chromatin remodeling at the molecular level. Three main targets are the current focus of our research: protein arginine methyltransferases (PRMTs), histone chaperones and histone deacetylases (HDACs). Furthermore, we are also developing structurebased rational approaches to characterize small molecule modulators of the biological activities of our targets since they are frequently involved in human diseases. Our team also participates in collaborative research on several related projects which require our specific expertise. Present collaborations concern the structural studies of complexes of cancer-related targets such as the Translationally Controlled Tumor Protein (TCTP) and the mRNA of human histone H4.
Members
Researchers
Engineers
Publications
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2017
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Structural studies of protein arginine methyltransferase 2 reveal its interactions with potential substrates and inhibitors
- Vincent Cura
- N Marechal
- N Troffer-Charlier
- Jean-Marc Strub
- M van Haren
- Nathaniel I. Martin
- Sarah Cianferani
- Luc Bonnefond
- Jean Cavarelli
FEBS J ; Volume: 284 ; Page: 77-96
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Transition state mimics are valuable mechanistic probes for structural studies with the arginine methyltransferase CARM1
- Matthijs van Haren
- Nils Marechal
- Nathalie Troffer-Charlier
- Agostino Cianciulli
- Gianluca Sbardella
- Jean Cavarelli
- Nathaniel Martin
Proceedings of the National Academy of Sciences of the United States of America ; Volume: 114 ; Page: 3625-3630
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Crystallization and Preliminary X-Ray Diffraction Analysis of a Mammal Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
- Elsa Franco-Echevarria
- Julia Sanz-Aparicio
- Nathalie Troffer-Charlier
- Arnaud Poterszman
- Beatriz Gonzalez
Protein Journal ; Volume: 36 ; Page: 240-248
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2015
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TCTP contains a BH3-like domain, which instead of inhibiting, activates Bcl-xL.
- Stéphanie Thébault
- Morgane Agez
- Xiaoke Chi
- Johann Stojko
- Vincent Cura
- Stéphanie B Telerman
- Laurent Maillet
- Fabien Gautier
- Isabelle Billas-Massobrio
- Catherine Birck
- Nathalie Troffer-Charlier
- Teele Karafin
- Joane Honoré
- Andrea Senff-Ribeiro
- Sylvie Montessuit
- Christopher M Johnson
- Philippe Juin
- Sarah Cianférani
- Jean-Claude Martinou
- David W Andrews
- ...
Scientific Reports ; Volume: 6 ; Page: 19725
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Insect Cells–Baculovirus System for the Production of Difficult to Express Proteins
- Judit Osz-Papai
- Laura Radu
- Wassim Abdulrahman
- Isabelle Kolb-Cheynel
- Nathalie Troffer-Charlier
- Catherine Birck
- A. Poterszman
Insoluble Proteins ; Volume: 1258 ; Page: 181-205
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Functional insights from high resolution structures of mouse protein arginine methyltransferase 6
- Luc Bonnefond
- J Stojko
- Justine Mailliot
- Nathalie Troffer-Charlier
- Vincent Cura
- Jean-Marie Wurtz
- S Cianferani
- Jean Cavarelli
Journal of Structural Biology ; Volume: 191 ; Page: 175-183
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Structure of the Elongator cofactor complex Kti11/Kti13 provides insight into the role of Kti13 in Elongator-dependent tRNA modification
- Olga Kolaj-Robin
- A Mcewen
- Jean Cavarelli
- Bertrand Séraphin
FEBS Journal ; Volume: 282 ; Page: 819-833
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2014
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Molecular basis for the antiparasitic activity of a mercaptoacetamide derivative that inhibits histone deacetylase 8 (HDAC8) from the human pathogen schistosoma mansoni
- D Stolfa
- Martin Marek
- J Lancelot
- A Hauser
- A Walter
- E Leproult
- J Melesina
- T Rumpf
- Jean-Marie Wurtz
- Jean Cavarelli
- W Sippl
- R Pierce
- Christophe Romier
- Matthieu Jung
Journal of Molecular Biology ; Volume: 426 ; Page: 3442-3453
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2013
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Structural Basis for the Inhibition of Histone Deacetylase 8 (HDAC8), a Key Epigenetic Player in the Blood Fluke Schistosoma mansoni
- Martin Marek
- Srinivasaraghavan Kannan
- Alexander-Thomas Hauser
- Marina Moraes Mourão
- Stéphanie Caby
- Vincent Cura
- Diana A Stolfa
- Karin Schmidtkunz
- Julien Lancelot
- Luiza Andrade
- Jean-Paul Renaud
- Guilherme Oliveira
- Wolfgang Sippl
- Manfred Jung
- Jean Cavarelli
- Raymond J Pierce
- Christophe Romier
PLoS Pathogens ; Volume: 9
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Structural Basis for Hijacking of Cellular LxxLL Motifs by Papillomavirus E6 Oncoproteins
- Katia Zanier
- Sebastian Charbonnier
- Abdellahi Ould M'Hamed Ould Sidi
- Alastair G Mcewen
- Maria Giovanna Ferrario
- Pierre Poussin-Courmontagne
- Vincent Cura
- Nicole Brimer
- Khaled Ould Babah
- Tina Ansari
- Isabelle Muller
- Roland H Stote
- Jean Cavarelli
- Scott Vande Pol
- Gilles Trave
Science ; Volume: 339 ; Page: 694-698
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