IGBMC : Institut de la Génétique et de la Biologie Moléculaire et Cellulaire

• Researchers

  Isabelle BILLAS MASSOBRIO

  Bruno KLAHOLZ

  Kundhavai NATCHIAR

  Alexandre OURJOUMTSEV

• Post-Doctoral Fellows

  Jaime ALEGRIO LOURO

  Leonid ANDRONOV

  Brice BEINSTEINER

  Vanessa DACLEU SIEWE

  Clara Ottilie LOEFFELHOLZ VON COLBERG

  Kareem MOHIDEEN ABDUL

• PhD Students

  Charles BARCHET

  Léo FRECHIN

  Samuel HOLVEC

  Pierre VILELA

• Engineers & Technicians

  Isabelle HAZEMANN

  Jean-François MENETRET

Integrated structural Biology

Large complexes involved in gene expression

Transcription and translation are fundamental molecular mechanisms of gene activity regulation with profound implications for human health. The ligand-dependent transcriptional regulation by nuclear receptors bound to DNA response elements involves the transient assembly of large co-regulator complexes. These trigger chromatin remodeling and facilitate the assembly of the general transcription machinery on the promoter of the target gene. Gene expression is also regulated at the level of protein synthesis, for example, by protein factors that bind to the ribosome during the translation initiation, elongation and termination phase. The initiation phase is strongly regulated by factors and also by the mRNA itself and well-characterized reaction intermediates of the initiating ribosomal nano-machinery are potential targets for antibiotics. Both transcription and translation complexes represent large, transient macromolecular assemblies that we investigate by using an integrative structural biology approach with crystallography and cryo-electron microscopy forming the core.

Bruno Klaholz is also scientific coordinator of the Biostructure structural biology research infrastructure, itself selected within the FRISBI project.

 Know more about the cryo electron microscopy facilities

Movie: Visualization of modifications in the human ribosome structure, a new feat for cryo electron microscopy

Job opportunities :

• Post-Doc

• Current projects

• Collaborations

• Prizes/Awards

  • Bruno Klaholz - Médaille d'Argent - CNRS - 2018
  • Bruno Klaholz - Raimond Castaing Prize - French Society of Microscopies - 2017
  • Bruno KLAHOLZ - Richard Lounsbery prize - Académie des sciences - 2016
  • Bruno KLAHOLZ - ERC Starting grant - European Research Council (ERC) - 2009
  • Bruno KLAHOLZ - Nuclear Receptor Conference Presentation Prize - European Molecular Biology Organization (EMBO) - 2009
  • Bruno KLAHOLZ - Bronze Medal - CNRS - 2008
  • Bruno KLAHOLZ - Paul Mandel Gutenberg Group Prize - CNRS - 2008
  • Bruno Klaholz - EMBO Young Investigator Award - European Molecular Biology Organization Young Investigator Programme (EMBO YIP) - 2006

• News

  • Sept. 13, 2018 - 2017, a year with the CNRS in Alsace
  • Nov. 15, 2017 - Visualization of modifications in the human ribosome structure, a new feat for cryo electron microscopy
  • April 22, 2015 - The fine structure of the human ribosome unveiled!
  • Nov. 7, 2014 - Focus on helical polyribosomes
  • June 19, 2014 - Interplay of symmetry and asymmetry in a nuclear receptor
  • Sept. 24, 2013 - Integrated approach in structural biology: initiation factor IF2 from every angle
  • Feb. 23, 2012 - Official launching of the ESFRI integrative structural biology infrastructure : INSTRUCT
  • Jan. 17, 2012 - First complete 3D mapping of vitamin D receptor

• Publications

  • Erratum: Ribosomal RNA 2' O-methylation as a novel layer of inter-tumour heterogeneity in breast cancer.

    Marcel V, Kielbassa J, Marchand V, Natchiar KS, Paraqindes H, Van Long FN, Ayadi L, Bourguignon-Igel V, Monaco PL, Monchiet D, Scott V, Tonon L, Bray SE, Diot A, Jordan LB, Thompson AM, Bourdon JC, Dubois T, André F, Catez F, Puisieux A, Motorin Y, Klaholz BP, Viari A, Diaz JJ

    NAR Cancer Mar 2021 ; 3:zcab006 .

  • Setup and Troubleshooting of Volta Phase Plate Cryo-EM Data Collection.

    von Loeffelholz O, Klaholz BP

    Methods Mol Biol 2021 ; 2305:291-299 .

  • Identification of Mg(2+) ions next to nucleotides in cryo-EM maps using electrostatic potential maps.

    Wang J, Natchiar SK, Moore PB, Klaholz BP

    Acta Crystallogr D Struct Biol April 1, 2021 ; 77:534-539 .

  • Nonsteroidal ecdysone receptor agonists use a water channel for binding to the ecdysone receptor complex EcR/USP.

    Browning C, McEwen AG, Mori K, Yokoi T, Moras D, Nakagawa Y, Billas IML

    J Pestic Sci Feb. 20, 2021 ; 46:88-100 .

  • Structural and dynamic characterization of the C-terminal tail of ErbB2: Disordered but not random.

    Pinet L, Wang YH, Deville C, Lescop E, Guerlesquin F, Badache A, Bontems F, Morellet N, Durand D, Assrir N, van Heijenoort C

    Biophys J May 18, 2021 ; 120:1869-1882 .

  • Myod1 and GR coordinate myofiber-specific transcriptional enhancers.

    Rovito D, Rerra AI, Ueberschlag-Pitiot V, Joshi S, Karasu N, Dacleu-Siewe V, Rayana KB, Ghaibour K, Parisotto M, Ferry A, Jelinsky SA, Laverny G, Klaholz BP, Sexton T, Billas IML, Duteil D, Metzger D

    Nucleic Acids Res May 7, 2021 ; 49:4472-4492 .

  • Practical Aspects of Super-Resolution Imaging and Segmentation of Macromolecular Complexes by dSTORM.

    Andronov L, Vonesch JL, Klaholz BP

    Methods Mol Biol 2021 ; 2247:271-286 .

  • Ribosomal RNA 2'O-methylation as a novel layer of inter-tumour heterogeneity in breast cancer.

    Marcel V, Kielbassa J, Marchand V, Natchiar KS, Paraqindes H, Nguyen Van Long F, Ayadi L, Bourguignon-Igel V, Lo Monaco P, Monchiet D, Scott V, Tonon L, Bray SE, Diot A, Jordan LB, Thompson AM, Bourdon JC, Dubois T, André F, Catez F, Puisieux A, Motorin Y, Klaholz BP, Viari A, Diaz JJ

    NAR Cancer Dec 2020 ; 2:zcaa036 .

  • Insights into the improved macrolide inhibitory activity from the high-resolution cryo-EM structure of dirithromycin bound to the E. coli 70S ribosome.

    Pichkur EB, Paleskava A, Tereshchenkov AG, Kasatsky P, Komarova ES, Shiriaev DI, Bogdanov AA, Dontsova OA, Osterman IA, Sergiev PV, Polikanov YS, Myasnikov AG, Konevega AL

    RNA Jun 2020 ; 26:715-723 .

  • Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction.

    Wang L, Myasnikov A, Pan X, Walter P

    Elife Jan. 30, 2020 ; 9: .

  See all publications=>

• Job opportunities

• Alumni

• Videos

  • Video human ribosome

• Thesis subjects