Integrated structural Biology
Large complexes involved in gene expression
Transcription and translation are fundamental molecular mechanisms of gene activity regulation with profound implications for human health. The ligand-dependent transcriptional regulation by nuclear receptors bound to DNA response elements involves the transient assembly of large co-regulator complexes. These trigger chromatin remodeling and facilitate the assembly of the general transcription machinery on the promoter of the target gene. Gene expression is also regulated at the level of protein synthesis, for example, by protein factors that bind to the ribosome during the translation initiation, elongation and termination phase. The initiation phase is strongly regulated by factors and also by the mRNA itself and well-characterized reaction intermediates of the initiating ribosomal nano-machinery are potential targets for antibiotics. Both transcription and translation complexes represent large, transient macromolecular assemblies that we investigate by using an integrative structural biology approach with crystallography and cryo-electron microscopy forming the core.
Bruno Klaholz is also scientific coordinator of the Biostructure structural biology research infrastructure, itself selected within the FRISBI project.
Know more about the cryo electron microscopy facilities
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Current projects
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Collaborations
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Prizes/Awards
- Bruno Klaholz - Médaille d'Argent - CNRS - 2018
- Bruno Klaholz - Raimond Castaing Prize - French Society of Microscopies - 2017
- Bruno KLAHOLZ - Richard Lounsbery prize - Académie des sciences - 2016
- Bruno KLAHOLZ - ERC Starting grant - European Research Council (ERC) - 2009
- Bruno KLAHOLZ - Nuclear Receptor Conference Presentation Prize - European Molecular Biology Organization (EMBO) - 2009
- Bruno KLAHOLZ - Bronze Medal - CNRS - 2008
- Bruno KLAHOLZ - Paul Mandel Gutenberg Group Prize - CNRS - 2008
- Bruno Klaholz - EMBO Young Investigator Award - European Molecular Biology Organization Young Investigator Programme (EMBO YIP) - 2006
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News
- Sept. 13, 2018 - 2017, a year with the CNRS in Alsace
- Nov. 15, 2017 - Visualization of modifications in the human ribosome structure, a new feat for cryo electron microscopy
- April 22, 2015 - The fine structure of the human ribosome unveiled!
- Nov. 7, 2014 - Focus on helical polyribosomes
- June 19, 2014 - Interplay of symmetry and asymmetry in a nuclear receptor
- Sept. 24, 2013 - Integrated approach in structural biology: initiation factor IF2 from every angle
- Feb. 23, 2012 - Official launching of the ESFRI integrative structural biology infrastructure : INSTRUCT
- Jan. 17, 2012 - First complete 3D mapping of vitamin D receptor
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Publications
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Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction.
Elife Jan. 30, 2020 ; 9: .
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Structural basis of nanobody recognition of grapevine fanleaf virus and of virus resistance loss
PNAS 2020 may 19 2020 ; : .
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Dynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes.
Cell Rep April 7, 2020 ; 31:107473 .
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Cells March 5, 2020 .
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Including crystallographic symmetry in quantum-based refinement: Q|R#2.
Acta Crystallogr D Struct Biol Jan. 1, 2020 .
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Acta Crystallogr D Struct Biol Oct. 1, 2019 .
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CENP-A nucleosome clusters form rosette-like structures around HJURP during G1.
Nat Commun Sept. 30, 2019 ; 10:4436 .
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Acta Crystallogr D Struct Biol Jan. 1, 2019 ; 75:79-89 .
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eIF2B-catalyzed nucleotide exchange and phosphoregulation by the integrated stress response.
Science May 3, 2019 ; 364:491-495 .
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Mol Cell March 21, 2019 ; 73:1217-1231 .
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