IGBMC : Institut de la Génétique et de la Biologie Moléculaire et Cellulaire

• Researchers

  Isabelle BILLAS MASSOBRIO

  Bruno KLAHOLZ

  Kundhavai NATCHIAR

  Alexandre OURJOUMTSEV

• Post-Doctoral Fellows

  Jaime ALEGRIO LOURO

  Leonid ANDRONOV

  Brice BEINSTEINER

  Vanessa DACLEU SIEWE

  Clara Ottilie LOEFFELHOLZ VON COLBERG

  Kareem MOHIDEEN ABDUL

• PhD Students

  Charles BARCHET

  Léo FRECHIN

  Samuel HOLVEC

  Pierre VILELA

• Engineers & Technicians

  Isabelle HAZEMANN

  Jean-François MENETRET

Integrated structural Biology

Large complexes involved in gene expression

Transcription and translation are fundamental molecular mechanisms of gene activity regulation with profound implications for human health. The ligand-dependent transcriptional regulation by nuclear receptors bound to DNA response elements involves the transient assembly of large co-regulator complexes. These trigger chromatin remodeling and facilitate the assembly of the general transcription machinery on the promoter of the target gene. Gene expression is also regulated at the level of protein synthesis, for example, by protein factors that bind to the ribosome during the translation initiation, elongation and termination phase. The initiation phase is strongly regulated by factors and also by the mRNA itself and well-characterized reaction intermediates of the initiating ribosomal nano-machinery are potential targets for antibiotics. Both transcription and translation complexes represent large, transient macromolecular assemblies that we investigate by using an integrative structural biology approach with crystallography and cryo-electron microscopy forming the core.

Bruno Klaholz is also scientific coordinator of the Biostructure structural biology research infrastructure, itself selected within the FRISBI project.

 Know more about the cryo electron microscopy facilities

Movie: Visualization of modifications in the human ribosome structure, a new feat for cryo electron microscopy

Job opportunities :

• Post-Doc

• Current projects

• Collaborations

• Prizes/Awards

  • Bruno Klaholz - Médaille d'Argent - CNRS - 2018
  • Bruno Klaholz - Raimond Castaing Prize - French Society of Microscopies - 2017
  • Bruno KLAHOLZ - Richard Lounsbery prize - Académie des sciences - 2016
  • Bruno KLAHOLZ - ERC Starting grant - European Research Council (ERC) - 2009
  • Bruno KLAHOLZ - Nuclear Receptor Conference Presentation Prize - European Molecular Biology Organization (EMBO) - 2009
  • Bruno KLAHOLZ - Bronze Medal - CNRS - 2008
  • Bruno KLAHOLZ - Paul Mandel Gutenberg Group Prize - CNRS - 2008
  • Bruno Klaholz - EMBO Young Investigator Award - European Molecular Biology Organization Young Investigator Programme (EMBO YIP) - 2006

• News

  • Sept. 13, 2018 - 2017, a year with the CNRS in Alsace
  • Nov. 15, 2017 - Visualization of modifications in the human ribosome structure, a new feat for cryo electron microscopy
  • April 22, 2015 - The fine structure of the human ribosome unveiled!
  • Nov. 7, 2014 - Focus on helical polyribosomes
  • June 19, 2014 - Interplay of symmetry and asymmetry in a nuclear receptor
  • Sept. 24, 2013 - Integrated approach in structural biology: initiation factor IF2 from every angle
  • Feb. 23, 2012 - Official launching of the ESFRI integrative structural biology infrastructure : INSTRUCT
  • Jan. 17, 2012 - First complete 3D mapping of vitamin D receptor

• Publications

  • Practical Aspects of Super-Resolution Imaging and Segmentation of Macromolecular Complexes by dSTORM.

    Andronov L, Vonesch JL, Klaholz BP

    Methods Mol Biol 2021 ; 2247:271-286 .

  • Insights into the improved macrolide inhibitory activity from the high-resolution cryo-EM structure of dirithromycin bound to the E. coli 70S ribosome.

    Pichkur EB, Paleskava A, Tereshchenkov AG, Kasatsky P, Komarova ES, Shiriaev DI, Bogdanov AA, Dontsova OA, Osterman IA, Sergiev PV, Polikanov YS, Myasnikov AG, Konevega AL

    RNA Jun 2020 ; 26:715-723 .

  • Structure of the AAA protein Msp1 reveals mechanism of mislocalized membrane protein extraction.

    Wang L, Myasnikov A, Pan X, Walter P

    Elife Jan. 30, 2020 ; 9: .

  • Structural basis of nanobody recognition of grapevine fanleaf virus and of virus resistance loss

    Igor Orlov 1 2 3 4 , Caroline Hemmer 5 6 , Léa Ackerer 5 6 7 , Bernard Lorber 8 , Ahmed Ghannam 5 , Vianney Poignavent 5 , Kamal Hleibieh 5 , Claude Sauter 8 , Corinne Schmitt-Keichinger 5 6 , Lorène Belval 6 , Jean-Michel Hily 6 7 , Aurélie Marmonier 6 , Véronique Komar 6 , Sophie Gersch 6 , Pascale Schellenberger 5 6 , Patrick Bron 9 , Emmanuelle Vigne 6 , Serge Muyldermans 10 , Olivier Lemaire 6 , Gérard Demangeat 6 , Christophe Ritzenthaler 11 , Bruno P Klaholz 12 2 3 4

    PNAS 2020 may 19 2020 ; : .

  • Dynamics of uS19 C-Terminal Tail during the Translation Elongation Cycle in Human Ribosomes.

    Bhaskar V(1), Graff-Meyer A(1), Schenk AD(1), Cavadini S(1), von Loeffelholz O(2), Natchiar SK(2), Artus-Revel CG(1), Hotz HR(1), Bretones G(3), Klaholz BP(2), Chao JA(4).

    Cell Rep April 7, 2020 ; 31:107473 .

  • Targeting the Human 80S Ribosome in Cancer: From Structure to Function and Drug Design for Innovative Adjuvant Therapeutic Strategies.

    Gilles A(1), Frechin L(2), Natchiar K(2), Biondani G(3), Loeffelholz OV(2), Holvec S(2), Malaval JL(1), Winum JY(1), Klaholz BP(2), Peyron JF(3).

    Cells March 5, 2020 .

  • Including crystallographic symmetry in quantum-based refinement: Q|R#2.

    Zheng M(1), Biczysko M(1), Xu Y(1), Moriarty NW(2), Kruse H(3), Urzhumtsev A(4), Waller MP(5), Afonine PV(2).

    Acta Crystallogr D Struct Biol Jan. 1, 2020 .

  • Deriving and refining atomic models in crystallography and cryo-EM: the latest Phenix tools to facilitate structure analysis.

    Klaholz BP(1).

    Acta Crystallogr D Struct Biol Oct. 1, 2019 .

  • CENP-A nucleosome clusters form rosette-like structures around HJURP during G1.

    Andronov L, Ouararhni K, Stoll I, Klaholz BP, Hamiche A

    Nat Commun Sept. 30, 2019 ; 10:4436 .

  • Mask-based approach to phasing of single-particle diffraction data. II. Likelihood-based selection criteria.

    Lunin VY(1), Lunina NL(1), Petrova TE(1), Baumstark MW(2), Urzhumtsev AG(3).

    Acta Crystallogr D Struct Biol Jan. 1, 2019 ; 75:79-89 .

  See all publications=>

• Job opportunities

• Alumni

• Videos

  • Video human ribosome

• Thesis subjects