Extending the reach of single-particle cryoEM

Abstract

Molecular structure determination using electron cryomicroscopy (cryoEM) will surpass X-ray crystallography as the most productive method for experimental determination of new structures during this coming year (2025), at least in terms of PDB depositions. Yet, there are still improvements to be made before we reach the physical limits set by radiation damage and the signal-to-noise ratio in images. I will give an overview of the current status with some examples and try to identify the remaining hurdles to making cryoEM even better.

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