Hybrid methods for analyzing conformational variability of biomolecules in cryo-EM and cryo-ET data

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Abstract:

The elucidation of different conformations of biomolecular complexes is the key to understanding their mechanisms in health and disease. Cryo electron microscopes allow 3D reconstruction of multiple molecular conformations after purification of the complexes and in their cellular environment. Since a few years, methods development to allow unravelling the entire conformational landscapes of the complexes from cryo electron microscopy data has been one of the most dynamic areas in the field. My group is developing such methods by combining image processing, molecular dynamics simulations, and deep learning approaches. These methods are publicly available via our open-source ContinuousFlex software package (a plugin of Scipion). In this talk, I will present our work regarding such methods for single particle analysis and cryo electron tomography.