Assembly, Fate and Architecture of cytoplasmic Nuclear Pore Complexes

Le February 25 2026 at 10h00 Séminaire

Nuclear Pore Complexes (NPCs) bridge across the nuclear envelope (NE) and mediate transport of macromolecules between the nucleus and the cytoplasm. NPCs consist of hundreds of nucleoporin building blocks and exemplify the structural complexity of macromolecular assemblies. Surprisingly, beyond their canonical localization at the nuclear surface, NPCs also populate subdomains of the ER termed Annulate Lamellae (AL). We combine imaging approaches with in situ structural biology to study the assembly, fate and architecture of NPCs at and beyond the nuclear envelope in the context of Drosophila development. In my presentation I will discuss how cytoplasmic NPCs emerge from condensed precursors during oogenesis and how this pool of pre-formed NPCs is used during the rapid division cycles in early embryos. I will focus on recent work in which we reveal the structure of NPCs outside the nuclear envelope, providing insight into the regulation of NPC (a)symmetry.