Molecular biology and virus
Molecular biology and virus service
The Molecular Biology and Virus Service realizes the construction of plasmids on demand and the conception, production and purification of viral vectors, in particular Lentiviral (retroviral) and Adeno-associated vectors.
CLONING: Thank to its plasmid backbones collection the service can construct plasmids for any kind of experimental need: expression in E.coli (different tags, resistance genes, replication origins), in baculovirus (different tags, expression of protein complexes), in mammalian cells, mutagenesis, CRISPR analysis (Cas9+1-4 gRNAs plasmids, homologous recombination plasmids), RNA interference, viral vectors construction.
VIRAL VECTORS: The service performs the production of lentiviral and adeno-associated vectors from the construction of the expression plasmid to the production, purification and titration of the viral particles: the lentiviral particles are produced at concentrations that can reach 109 TU/ml and can be used to modify non-transfectable cells, to construct stable cell lines or realize a high throughput CRISPR screening; the adeno-associated vectors of different serotypes are produced at concentrations reaching 1013 GC/ml and are used for in vivo studies in mice, to overexpress, inactivate (shRNA, miRNA, CRISPR knock-out) or modify genes.