Contact

Protéomics

Protéomics

PLATFORM LEADER

Luc NEGRONI

The IGBMC Proteomics Platform specializes in the identification and quantification of proteins and proteomes. It relies on a team expert in mass spectrometry, peptide synthesis and bioinformatics to meet the analytical needs of academic or private partners.

Expertise

  • Peptide synthesis
  • Protein / proteome analysis in solution or in gel
  • Characterization of post-translational modifications (phospho-STY, acetyl-K, ubi-K, Glyco-N)
  • Protein quantification (label free, SILAC, TMT, iTRAQ)
  • Molecular mass verification of purified proteins
  • Structural study (SS bridges, XL-MS)
  • Bioinformatics and statistical analysis (Perseus, R, python)
  • Carrying out studies and R&D developments, collaboration

Former members

  • Manuela Argentini : 2001-2007
  • Ingrid Colas : 2005-2006
  • Adeline Page : 2009-2014
  • Virginie Chavant : 2011-2014
  • Mathilde Joint : 2013-2016
  • Mathilde Decourcelle : 2015-2016
  • Bastien Lefeuvre : 2019
  • Sangeetha Covindarassou : 2018
  • Kim Barosso: 2017-2019

Current projects

  • Nuclear transcription complexes analysis (col. Laszlo Tora, Illkirch)
  • Unfolding Protein Response of IRE1 and protein homeostasis (col.  Eric Chevet, Rennes)
  • Comparison, benchmarking and dissemination of proteomics data analysis pipelines (ELIXIR organization)
  • XL-MS analysis of IKKbeta kinase subunit (col. Katia Zanier, Illkirch)

 

 

 

Collaborations and networks

  • Irene Bozzoni, Non coding RNAs in Physiology and Pathology , Sapienza Univ. Rome
  • Eric Chevet, Chemistry Oncogenesis Stress Signaling, U1242, Rennes
  • Mike Osta, Department of Biology, American University of Beirut,Beirut
  • Katia Zanier, Nuclear signaling and cancer, Biotechnology and cell signaling, UMR7242 Illkirch

Funding and partners

  • Association pour la Recherche sur le Cancer ARC
  • ITMO Cancer INSERM

Resources

  • Orbitrap Elite and nanoLC nano chromatography system
  • QToT Xevo XS G1 and nanochromatography system
  • SCX-HPLC and collecting system
  • Processing : Proteome Discoverer, Skyline, MaxQuant, Perseus, R