Phage and Yeast united for the better: Synthetic Nanobody® selection and optimization

Le October 09 2023 at 11h00 Séminaire

Phage display nanobody selection from our fully synthetic humanized library is a reality with more than hundred successful projects since the publication of our library developed with Sandrine Moutel, Aurélien Olichon and Franck Perez¹.

We selected with our colleagues and customers VHHs against unstructured proteins ², peptide tag³, various soluble proteins⁴, small molecules¹ or challenging DNA structures⁵.

Building from our 25 years of experience in Yeast genetic, we developed a yeast-two-hybrid and a Yeast display platform for intrabodization, and optimization of nanobodies coming from our library or from the lama⁶.

Working fluently between phage and yeast world, we can address many complex projects particularly when there is bottleneck in antigen expression or purification. We will illustrate that screening of naïve yeast library is also a very powerful alternative to find intracellularly functional VHHs against soluble antigens.

We will present new results around nanobodies active against TAU aggregation, nanobody against V5 tag and Ubiquitin and chain.

1 Moutel S. et al. Elife 2016, 19 : 5 ; 2 Danis C. et al, Mol. Ther. 2022 6;30(4):1484-1499. ; 3 Zeghal M et al. J Biol Chem. 2023 Jul 28;105107; 4 Jang H et al, Mol Immunol. 2020 Feb; 118:110-116 (among others); 5 Galli S. et al, J. Am. Chem. Soc. 2022, 144, 50, 23096–23103; 6 Dupré E. et al. ACS Chem Neurosci. 2019 Sep 18;10(9):3997-4006.; 7 Gonzalez-Santamarta M, et al., Methods Mol Biol. 2023;2602:125-136.

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