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PluriCell East

PluriCell East

PLATFORM LEADER

Amélie FREISMUTH

With more than 30 years of expertise in cell culture, the PluriCell East platform provides scientific and technological services and support tailored to the needs of academic and private research communities, both in France and internationally.

PluriCell East maintains a referenced collection of more than 2,400 human and animal cell lines, including normal and tumor cell lines, as well as cell lines derived from patients with genetic diseases. The platform provides services including the culture and storage of primary cells and human and animal cell lines, large-scale suspension cell culture in volumes of up to 25 L, and the production and preparation of culture media. We also provide quality control services and testing of new culture conditions, for example when evaluating new FCS batches or culture media.

These activities rely on rigorous monitoring and management of cell cultures. Cell lines and cultures are registered in our database and are subject to continuous monitoring and traceability throughout their use. Established protocols and procedures are applied to the different stages of cell culture and handling to ensure reproducible practices, reliable project monitoring, and consistent quality of our services.

For more than 10 years, the platform has developed specific expertise in the culture, maintenance, and characterization of pluripotent stem cells, particularly human induced pluripotent stem cells (hiPSCs) and mouse embryonic stem cells (mESCs).

This expertise has been extended to CRISPR-Cas9-based genome editing of hiPSCs. Since the launch of this service in spring 2025, 7 hiPSC genome editing projects have already been completed, including knock-out (KO), knock-in (KI), and base editing approaches. The platform has also recently developed a somatic cell reprogramming service to generate hiPSCs.

 

 

 

Our services

The PluriCell East platform provides its expertise as well as scientific and technological support to meet users’ needs.

Cell Culture

  • Culture and storage of primary cells and human and animal cell lines, including normal and patient-derived cells.
  • Large-scale suspension cell culture, with volumes of up to 25 L.
  • Production of LIF (Leukemia Inhibitory Factor).
  • Production and preparation of customized culture media.

Pluripotent Stem Cells

  • Culture, maintenance, and storage of human induced pluripotent stem cells (hiPSCs) and mouse embryonic stem cells (mESCs).
  • CRISPR-Cas9-based genome editing of hiPSCs, including KO, KI, and base editing approaches.
  • Reprogramming of somatic cells into hiPSCs.NEW
  • Training of users in hiPSCs culture.
  • Access to dedicated BSL-2 laboratories for hiPSCs culture.

Cell Culture Quality Control

PluriCell East provides a range of quality control and cell culture characterization services, including:

  • Thawing tests
  • Mycoplasma detection
  • hiPSC characterization
  • Karyotyping and chromosome counting

Optimization and Development of Culture Conditions

The platform provides cell culture condition optimization and testing of new products or methods, including materials, culture media, serum batches, and optimization of cell culture techniques.

Project Support

PluriCell East also provides technical and scientific support to researchers throughout their projects.

BSL-2 Laboratories available to users

  • 2 BSL-2 laboratories dedicated to hiPSCs culture.
  • 1 BSL-2 laboratory dedicated to virus handling.

New

  • Somatic Cell Reprogramming into hiPSCs 

PluriCell East has developed a new somatic cell reprogramming service for the generation of human induced pluripotent stem cells (hiPSCs).

A first pilot project was carried out using two fibroblast cultures, enabling the platform to establish and validate the experimental conditions required to implement this new service.

 

 

 

 

 

 

 

Ressources

Access restricted to the platform’s members

 

 

120 m² cell culture laboratories (L1 et L2)

 

  • 9 Biological Safety Cabinets HERASAFE KS12 et KS15
  • 12 Incubators CO2 Panasonic
  • 3 inverted Zeiss Microscopes
  • 2 observation system Lynx Stereo Vision Engineering for hiPSC culture
  • 1 NucleoCounter NC-202 ChemoMetec
  • 1 room at 37°C for large scale suspension cells culture

Cryogenic room

  • 7 Cryocontainer CF 320M – 350 L – Cryodiffusion
  • 1 Freezer -86°C SANYO

 

 

 

Laverie dédiée

  • 2 Washers - Lancer
  • 2 Ovens Memmert (UF750 et UFE600)
  • 1 Autoclave DE LAMA
  • 1 Autoclave MATACHANA S1000

 

 

 

 

Laboratories accessible to the platform’s users
 

Laboratory L2 dedicated for experiment with virus:

  • 6 Biological Safety Cabinets HERASAFE KS12
  • 7 incubators CO2 Panasonic
  • Evos Fluorescence Microscope
  • Ultra-centrifuge Beckman
  • 1 Freezer -86°C SANYO

2 Laboratories L2 dedicated to hiPSC culture:

  • 7 Biological Safety Cabinets HERASAFE KS12 et KS15
  • 6 incubators CO2 Panasonic
  • 1 incubator tri-gas for culture in hypoxia
  • 4 Leica observation systems
  • 2 Lynx observation system Stereo Vision Engineering
  • 1 inverted Microscopes whose 1 fluorescence microscope with camera

 

 

 

 

Access

For any request to the PluriCell East Platform, please contact us at :
pluricell-east@igbmc.fr

Contribution of platform’s

In your publication acknowledgments, please mention the contribution of platform’s expertise with the following sentence :
« We thank the PluriCell East platform for cell culture services »

Networks

The PluriCell East platform is involved in several regional, national and European networks :

 

 

 

 

 

Fundings and Partners

 

 

 

 

Engineers

Technicians

Publications