Chromatin stability and DNA mobility
Team Leaders : Valerie LAMOUR , Marc RUFF
Department : Integrated structural biology
We investigate the regulation of DNA topology by DNA topoisomerases that form large nucleoprotein complexes and catalyze relaxation of DNA entanglements in bacteria and human cells.
Our project is aiming at the functional and structural studies of DNA topoisomerase cellular complexes targeted by therapeutic compounds used in cancer and anti-bacterial therapies. The main goal of this study is to identify and study the structure/function of Topoisomerase-associated complexes targeted by drugs through a combination of functional and structural approaches.
- Structural analysis of chromatin complexes of human DNA topoisomerases (TopoII).
- Impact of post-translational modifications on the catalytic activities of the human Topo II isoforms.
- Structure/function analysis of nucleoproteic complexes of the bacterial DNA gyrase.
- Interplay of DNA topology and transcription (in collaboration with the team of Albert Weixlbaumer at IGBMC)
Team Leaders : Valerie LAMOUR , Marc RUFF
Department : Integrated structural biology
Life Science Alliance ; Volume: 5 ; Page: e202201568
Nature Communications ; Volume: 12
Science Advances ; Volume: 7 ; Page: eabe0384
Biophysical Journal ; Volume: 118 ; Page: 532a
Cancer Drug Resistance ; Page: 149-160
Nature Communications ; Volume: 10 ; Page: 4935
Scientific Reports ; Volume: 8
Journal of Medicinal Chemistry ; Volume: 62 ; Page: 4225-4231
Médecine/Sciences ; Volume: 34 ; Page: 462-463
The Bacterial Nucleoid. Methods and Protocols ; Page: 193-210