Congratulations to Alexia Kindler on the acceptance of her thesis!
Congratulations to Alexia Kindler for the acceptance of her thesis! Her work was directed by Wojciech Krezel and is titled: "Deciphering retinoid metabolic and signaling pathways using bio-orthogonal chemistry-based strategies".
At interface between biology and chemistry thanks to a collaboration with chemists from Dr Alain Wagner team (Jessica Coulleray), Alexia Kindler developed an innovative method for the detection of modified retinoids in biological matrices. Using a Click chemistry approach to better understand their metabolism and storage in the brain, her work allowed to eliminate unsuitable fluorescent probes for this detection on neuronal cells. On the other hand, she obtained promising results with mass probes for detection in biological tissues. As part of a secondary project, she also validated an in vitro model of neural cells to study neurodevelopmental disorders. With this in vitro model, she was able to provide preliminary results for the study of RARß involvement in neural cells differentiation. This model will be used to study a rare neurodevelopmental disorder (MCOPS12), resulting from RARß mutation.
An article is currently in preparation for this work, it should be available online soon!
During her time at IGBMC, Alexia Kindler developed crucial skills in microscopy and cell culture, specifically for primary neural cell culture.
Alexia Kindler’s thesis was made possible through the support of our health and biology platforms Flux cytometry and Cell culture. The PhD student also strongly collaborated with our Photonic microscopy (Erwan Grandgirard, Elvire Guiot) and ICS platforms.
Alexia Kindler is now looking for a new position in neurobiology to keep developing her skills in primary cell cultures, with a particular interest in neurodegenerative disorders