Sorters
Contact
To request a service, simply fill out the contact form and send it to us at groupe-tricel@igbmc.fr.
Sorting speed
How long does it take me to sort?
- For most primary cells: A 70µm nozzle will be used and the average sorting speed is 50 x 106 cells per hour.
- For cultured cell lines: a 100µm nozzle should be used. The average sorting speed will be 10 x 106 cells per hour.
Example:
If you want to sort a population expressed at 5% by the sample at the beginning, the viability being 90%, you would like to recover more than 106 cells, it will be necessary to have 40 x106 cells at the beginning and the sorting will last about 2 hours minimum.
In any case, you must also add 45 minutes for the preparation time of the machine and the washing time.
Please note:
4 distinct populations can be recovered at the same time on Becton Dickinson sorters.
2 distinct populations can be recovered with the Bio-rad sorter.
N.B. It is imperative to filter all samples before sorting!
Information for cell sorting
Controls required for a sort
- Control of unlabeled cells
- Negative control
- Compensating checks if necessary
Sample sorting
Place samples in sterile 12 x 75 mm stoppered tubes (polypropylene or polystyrene tubes), Eppendorf or Falcon 15ml with maximum 5% serum.
A volume of 500µL is sufficient for the controls, with a minimum of 300,000 cells.
Samples should be resuspended at a concentration of 10-50 x106 cells/ml.
Cell harvesting tubes
(4 separate populations can be recovered at the same time).
For each tube, fill to ¼ full with the appropriate recovery medium.
You can use 12x75mm, Eppendorf or Falcon 15 ml tubes.
Cells sorted with a 70 micron nozzle are at a concentration ~106 cells/ml.
Cells sorted with a 1000 micron nozzle are at a concentration ~250,000 cells/ml.
Remember to bring back enough collection tubes for each population.
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