Three high-speed sorters with different optical configurations

Three high speed sorters are available to users. They allow to collect 4 populations simultaneously and to clone in 96 or 384 well plates. It is also possible to sort under sterile conditions.


5 lasers : 355 nm, 405 nm, 488 nm, 561 nm, 633 nm.

15 parameters

Cloning module

BioRad S3

2 lasers : 488 nm, 561 nm.

4 parameters








4 lasers : 405 nm, 488 nm, 561 nm, 633 nm.

16 parameters

Cloning module

Biosafety cabinet

Aria FUSION 2022

5 Lasers: 355nm-405nm-488nm-561nm-640nm
18 Parameters




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Sorting speed

How long does it take me to sort?

  • For most primary cells: A 70µm nozzle will be used and the average sorting speed is 50 x 106 cells per hour.   
  • For cultured cell lines: a 100µm nozzle should be used. The average sorting speed will be 10 x 106 cells per hour.   


If you want to sort a population expressed at 5% by the sample at the beginning, the viability being 90%, you would like to recover more than 106 cells, it will be necessary to have 40 x106 cells at the beginning and the sorting will last about 2 hours minimum.
In any case, you must also add 45 minutes for the preparation time of the machine and the washing time.

Please note:

4 distinct populations can be recovered at the same time on Becton Dickinson sorters.
2 distinct populations can be recovered with the Bio-rad sorter.

N.B. It is imperative to filter all samples before sorting!

Information for cell sorting

Controls required for a sort

  1. Control of unlabeled cells
  2. Negative control
  3. Compensating checks if necessary

Sample sorting

Place samples in sterile 12 x 75 mm stoppered tubes (polypropylene or polystyrene tubes), Eppendorf or Falcon 15ml with maximum 5% serum.
A volume of 500µL is sufficient for the controls, with a minimum of 300,000 cells.

Samples should be resuspended at a concentration of 10-50 x106 cells/ml.

Cell harvesting tubes

(4 separate populations can be recovered at the same time).
For each tube, fill to ¼ full with the appropriate recovery medium.
You can use 12x75mm, Eppendorf or Falcon 15 ml tubes.

Cells sorted with a 70 micron nozzle are at a concentration ~106 cells/ml.
Cells sorted with a 1000 micron nozzle are at a concentration ~250,000 cells/ml.
Remember to bring back enough collection tubes for each population.

Translated with (free version)

After Sorting

To optimize cell yield, fill the collection tubes and centrifuge.