Structure-fonction of nucleoproteic complexes of DNA topoisomerases
Structure-fonction of nucleoproteic complexes of DNA topoisomerases
We investigate the regulation of DNA topology by DNA topoisomerases that form large nucleoprotein complexes and catalyze relaxation of DNA entanglements in bacteria and human cells.
Our project is aiming at the functional and structural studies of DNA topoisomerase cellular complexes targeted by therapeutic compounds used in cancer and anti-bacterial therapies. The main goal of this study is to identify and study the structure/function of Topoisomerase-associated complexes targeted by drugs through a combination of functional and structural approaches.
Current projects
- Structural analysis of chromatin complexes of human DNA topoisomerases (TopoII).
- Impact of post-translational modifications on the catalytic activities of the human Topo II isoforms.
- Structure/function analysis of nucleoproteic complexes of the bacterial DNA gyrase. Check our latest article in Science 2024 : here
- Interplay of DNA topology and transcription (in collaboration with the team of Albert Weixlbaumer at IGBMC)
Awards and recognitions
Check our latest article published in Science, April 12th 2024 !
https://www.science.org/stoken/author-tokens/ST-1818/full
DNA gyrase captures a positive crossover prior to taking action.
Members
Researchers
PhD students
Publications
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2017
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A User-Friendly DNA Modeling Software for the Interpretation of Cryo-Electron Microscopy Data.
- Damien Larivière
- Rodrigo Galindo-Murillo
- Eric Fourmentin
- Samuel Hornus
- Bruno Lévy
- Julie Giraud
- Jean-François Menetret
- Valerie Lamour
The Bacterial Nucleoid. Methods and Protocols ; Page: 193-210
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A no-stop mutation in MAGEB4 is a possible cause of rare X-linked azoospermia and oligozoospermia in a consanguineous Turkish family
- Ozlem Okutman
- Jean Muller
- Valerie Skory
- Jean Marie Garnier
- Angeline Gaucherot
- Yoni Baert
- Valérie Lamour
- Munevver Serdarogullari
- Meral Gultomruk
- Albrecht Röpke
- Sabine Kliesch
- Viviana Herbepin
- Isabelle Aknin
- Moncef Ben Khalifa
- Marius Teletin
- Emre Bakircioglu
- Ellen Goossens
- Nicolas Charlet-Berguerand
- Mustafa Bahceci
- Frank Tüttelmann
- ...
Journal of Assisted Reproduction and Genetics ; Volume: 34 ; Page: 683-694
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2010
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A chemical labeling strategy for proteomics under nondenaturing conditions
- Ghyslain Budin
- Martin Moune Dimala
- Valérie Lamour
- Pierre Oudet
- Charles Mioskowski
- Stephane Meunier
- Laurent Brino
- Alain Wagner
ChemBioChem ; Volume: 11 ; Page: 79-82
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Nondenaturing chemical proteomics for protein complex isolation and identification
- Ghyslain Budin
- Martin Moune-Dimala
- Geoffray Leriche
- Jean-Michel Saliou
- Julie Papillon
- Sarah Sanglier-Cianferani
- Alain van Dorsselaer
- Valérie Lamour
- Laurent Brino
- Alain Wagner
ChemBioChem ; Volume: 11 ; Page: 2359-2361
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Computational tools in protein crystallography.
- Deepti Jain
- Valerie Lamour
Methods in Molecular Biology ; Volume: 673
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2009
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Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex.
- Valerie Lamour
- Lars F. Westblade
- Elizabeth A. Campbell
- Seth A. Darst
Journal of Structural Biology ; Volume: 168 ; Page: 352-6
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2004
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Proteolytic cleavage of the hyperthermophilic topoisomerase I from Thermotoga maritima does not impair its enzymatic properties
- Raynald Cossard
- Thierry Viard
- Valérie Lamour
- Michel Duguet
- Claire Bouthier de La Tour
BBA - Biochimica et Biophysica Acta ; Volume: 1700 ; Page: 161-170
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Ultrahigh resolution drug design I: details of interactions in human aldose reductase-inhibitor complex at 0.66 A
- E Howard
- R Sanishvili
- Raul E. Cachau
- André Mitschler
- B Chevrier
- P Barth
- Valérie Lamour
- M van Zandt
- E Sibley
- C Bon
- Dino Moras
- Thomas R. Schneider
- Andrzejj Joachimiak
- Alberto Podjarny
Proteins ; Volume: 55 ; Page: 792-804
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2003
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ATP-bound conformation of topoisomerase IV: a possible target for quinolones in Streptococcus pneumoniae
- Farid Sifaoui
- Valérie Lamour
- Emmanuelle Varon
- Dino Moras
- Laurent Gutmann
Journal of Bacteriology ; Volume: 185 ; Page: 6137-6146
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2002
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Crystallization of the 43 kDa ATPase domain of Thermus thermophilus gyrase B in complex with novobiocin
- Valérie Lamour
- L. Hoermann
- Jean-Marc Jeltsch
- Pierre Oudet
- Dino Moras
Acta Crystallographica Section D: Biological Crystallography ; Volume: 58 ; Page: 1376-1378
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