
In vivo cellular plasticity and direct reprogramming
In vivo cellular plasticity and direct reprogramming
There is more to cells than meets the eye !!! While most differentiated cells maintain their specialized identity their whole life, some change their identity and become something else: this is fascinating !!! We focus on direct reprogramming, the process where a differentiated cell swaps its identity to become another kind of differentiated cell (aka Transdifferentiation; check out our Lambert at al, 2021 article for a more philosophical discussion of cellular identity).
How can this happen, and especially how can this happen naturally in a range of species ? Numerous examples of cellular plasticity in physiological, experimental and pathological settings exist, but precisely how a differentiated cell can change its identity remains unclear. Work in the lab aims at exploring the many questions triggered by such phenomenon : What enables a cell to change it its identity and what steps and cellular trajectory are at play ? How similar are different reprogramming processes ? Does this happen only in specific cells ? Are there mechanisms to suppress reprogramming in other cells ? How is this influenced by the environment ?
To tackle these important questions we established a powerful and innovative model, looking at natural direct reprogramming events in vivo, at the single cell level, in the nematode C. elegans. For example, we have dissected how a rectal cell changes its identity into a moto-neuron. Because of the C. elegans invariant cellular lineage, our system gives us access to all the steps of the process, including the early ones, a unique asset. We have begun the systematic identification of the molecular networks and the dissection of the cellular requirements underlying several direct cell type conversion in vivo, and use a combination of genetics (mutants and RNAi), imaging, genome recombineering (CRISPR-Cas9), biochemical and transcriptomics (single cell RNA Seq) approaches.
Our integrated approaches will contribute to unravel not only key mechanisms that allow a differentiated cell to become plastic and change its identity, but also fundamental principles governing cellular identity maintenance & reprogramming. Such knowledge has significant therapeutic implications, as it will deepen our understanding of the initiation of certain cancers, and will improve our ability to reprogram cells for regenerative medicine purposes.
You would like to solve the next questions in cellular plasticity ? We welcome spontaneous applications all year round (sophie@igbmc.fr)
Members
Researchers
Post-doctoral fellows
PhD students
Trainees
Funding and partners
Work in the lab has benefited from the following fundings:
- ERC Consolidator Grant PlastiCell
- COST Grant BM1408 (Chair)
- ANR
- EMBO YIP networking grant / european GENiE network
- Ligue Nationale Contre le Cancer (LNCC)
- Association Française contre les Myopathies «AFM pôle IGBMC »
- EuroSyStem European Network of excellence
- Fondation pour la Recherche Médicale (FRM)
- Association pour la Recherche contre le Cancer (ARC)
- Association Française contre les Myopathies (AFM)
- ATIP de Biologie Cellulaire and ATIP+ de Biologie Cellulaire (CNRS)
News

Does dividing change a cell's chances of being reprogrammed?
Transdifferentiation is the conversion of one fully differentiated cell type into another. In a study published in Cell Reports, scientists…
Read more
Awards and recognitions
- ERC CoG grant
- Nominated to the AcademiaNet (by EMBO)
- Grand Prix pour la Recherche Fondamentale, Académie des Sciences / Fondation Générale de Santé
- EMBO YIP (Young Investigator Programme) Award
- EuroSyStem Young Investigator Award
- Prix Scientifique de la Fondation Schlumberger Pour la Recherche et l’Enseignement (FSER)
- Prix Scientifique de la FRM
- « Initiative postdoc - retour », Ministère de la Recherche, des Sciences et de l'Enseignement
Resources
Our team is a founding member of the European Network GENiE https://worm-genie.eu/
- Watch Marie Charlotte, former PhD student, explain her project in 3 min (French)
- Watch a presentation of our team research for the celebration of the 10 years of the ERC http://www.canalc2.tv/video/14415
- Our open access review on cellular plasticity in the worm https://www.genetics.org/content/213/3/723.full
- WormBook http://wormbook.org/
- WormBase https://wormbase.org/
- WormAtlas https://www.wormatlas.org/
- Our open access articles:
- Riva et al 2021 https://doi.org/10.1101/2021.05.05.442643
- Ahier et al 2020 https://doi.org/10.17912/micropub.biology.000339
- Gomez-Saldivar et al 2020 https://doi.org/10.1534/genetics.120.303774
- Morin et al 2020 https://www.micropublication.org/journals/biology/micropub-biology-000218/
- Vibert et al 2018 http://www.ijdb.ehu.es/web/paper/180123sj/wound-healing-cellular-regeneration-and-plasticity-the-elegans-way
- Götz & Jarriault 2017 https://journals.biologists.com/dev/article/144/15/2714/48056/Programming-and-reprogramming-the-brain-a-meeting
- Doitsidou et al 2016 https://www.genetics.org/content/204/2/451.short
- Becker & Jarriault 2016 https://www.sciencedirect.com/science/article/pii/S0959437X16300892
- Zuryn et al 2014 https://science.sciencemag.org/content/345/6198/826
- Ahier & Jarriault 2014 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3948794/
- Zuryn & Jarriault 2013 https://www.tandfonline.com/doi/full/10.4161/worm.25081
- Kagias et al 2012 https://www.pnas.org/content/109/17/6596.long
- Morin & Jarriault 2011 https://www.medecinesciences.org/fr/articles/medsci/full_html/2011/03/medsci2011273p331/medsci2011273p331.html
- Richard et al 2011 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3188571/
- Zuryn et al 2010 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2940307/
- Jarriault et al 2008 https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2268801/