
Nuclear organisation and division
Nuclear organisation and division
Living cells have a fascinating ability to generate complex and dynamic internal structures. Nowhere is this property more evident than during cell division: in only a few minutes, cells alter their shape, duplicate and partition their internal components, and divide into two apparently identical halves, which in many cases go on to acquire distinct identities. These dramatic changes need to be carefully coordinated with each other in space and time.
To gain insight into these processes, we focus on two major cell cycle transitions. First, we study how chromosome segregation and cytokinesis are coordinated with each other. In particular, we study how the Aurora-B-dependent “NoCut” checkpoint regulates cytokinesis abscission when chromatin bridges are delayed in the cell division site. Second, we are interested in mechanisms that establish differences in nuclear organization after asymmetric cell division, and in how nuclear pore complexes control the establishment of cell identity.
Members
Researchers
Former members
- Gabriel Neurohr (Group Leader at ETH Zurich)
- Iris Titos (Research Fellow, Department of Neurobiology, Harvard Medical School)
- Nuno Amaral (Technical Writer, Sweden)
- Aina Masgrau (Senior Validation Consultant, Spain)
- Andrea Battola (Medical Affairs Manager, Italy)
- Michael Maier (Postdoctoral Researcher, Institute of Medical Biology, Singapore)
- Francesca Di Giovanni (Technical Support Scientist, France)
- Tsvetomira Ivanova
- Arun Kumar (Postdoctoral Researcher, Cell and Molecular Biology Program, Pompeu Fabra University, Spain)
- Petra Stockinger (Research and Development Specialist, Spain)
- Zhanna Shcheprova (Senior Data Team Lead, France)
- Anne Daulny
- Trinidad Sanmartin (laboratory technician, Institute for Bioengineering of Catalonia, Spain)
- Mercè Gomar (Researcher Juan de la Cierva, University of Valencia, Spain)
- Céline Birling (ingénieur d'études, France)
Current projects
- Molecular characterization of the NoCut checkpoint in normal and cancer cells
- Control of Cell Identity by Nuclear Pore Acetylation
Funding and partners
- Ligue Nationale Contre le Cancer
- Fondation ARC
- Université de Strasbourg, Institute for Advanced Studies
- Fondation pour la Recherche Médicale
Awards and recognitions
European Research Council, Starting Grant (2011)
Publications
2017
DNA replication stress: NoCut to the rescue
- Nuno Amaral
- Nicola Brownlow
- Manuel Mendoza
Cell Cycle ; Volume: 16 ; Page: 233-234
2016
The Aurora-B-dependent NoCut checkpoint prevents damage of anaphase bridges after DNA replication stress
- Nuno Amaral
- Alexandre Vendrell
- Charlotta Funaya
- Fatima-Zahra Idrissi
- Michael Maier
- Arun Kumar
- Gabriel Neurohr
- Neus Colomina
- Jordi Torres-Rosell
- María-Isabel Geli
- Manuel Mendoza
Nature Cell Biology ; Volume: 18 ; Page: 516-526
Time-Lapse Fluorescence Microscopy of Budding Yeast Cells
- Arun Kumar
- Manuel Mendoza
Yeast Cytokinesis ; Volume: 1369 ; Page: 1-8
2014
Chromosome length and perinuclear attachment constrain resolution of DNA intertwines
- Iris Titos
- Tsvetomira Ivanova
- Manuel Mendoza
Journal of Cell Biology ; Volume: 206 ; Page: 719-733
2011
A Midzone-Based Ruler Adjusts Chromosome Compaction to Anaphase Spindle Length
- Gabriel Neurohr
- Andreas Naegeli
- Iris Titos
- Dominik Theler
- Basil Greber
- Javier Díez
- Toni Gabaldón
- Manuel Mendoza
- Yves Barral
Science ; Volume: 332 ; Page: 465-468
2009
A mechanism for chromosome segregation sensing by the NoCut checkpoint
- Manuel Mendoza
- Caren Norden
- Kathrin Durrer
- Harald Rauter
- Frank Uhlmann
- Yves Barral
Nature Cell Biology ; Volume: 11 ; Page: 477-483
2008
Cytokinesis: Keeping Ring and Membrane Together
- Manuel Mendoza
- Yves Barral
Current Biology - CB ; Volume: 18 ; Page: R479-R480
Co-ordination of cytokinesis with chromosome segregation
- Manuel Mendoza
- Yves Barral
Biochemical Society Transactions ; Volume: 36 ; Page: 387-390
2006
The NoCut Pathway Links Completion of Cytokinesis to Spindle Midzone Function to Prevent Chromosome Breakage
- Caren Norden
- Manuel Mendoza
- Jeroen Dobbelaere
- Chitra Kotwaliwale
- Sue Biggins
- Yves Barral
Cell ; Volume: 125 ; Page: 85-98
2005
The fission yeast MO25 protein functions in polar growth and cell separation
- Manuel Mendoza
- Stefanie Redemann
- Damian Brunner
European Journal of Cell Biology ; Volume: 84 ; Page: 915-926