Transcription initiation machinery variability and functionality during differentiation and development
Subgroup Leader : Stéphane VINCENT
Over the past years our lab has identified and characterized a large number of metazoan nuclear transcription factors. These regulatory proteins include RNA polymerase II initiation factors (TFIID in particular) and co-activators (Spt-Ada-Gcn5 acetyl transferase, SAGA; or Ada Two A containing; ATAC) that form large multi-subunit complexes at promoter DNA to mediate transcription initiation and/or modify the epigenetic landscape of the genome. Our studies demonstrate that these complexes or their variants play a critical role in regulating transcription, as well as chromatin-regulatory processes. Our main research interest is to study the nature and assembly of these molecular machineries, the mechanisms by which they control how specific protein coding genes are turned on and off in the nucleus of a given cell, during growth, differentiation and development. We use biochemical, genetic, cell biology, imaging, biophysical, bioinformatic and structural approaches to study the control of gene expression, genome stability and chromatin architecture in different eukaryotic organisms (yeast, mouse and human). Our current and planned activities aim to better understand gene regulatory processes in different cellular systems and during the development of intact vertebrate organisms. We are also interested how does the disruption of this highly regulated processes lead to various disease states.
1) Study the assembly of transcription complexes;
2) Analysis of preinitiation complex variability and their role in transcription regulation during development and differentiation (during oocyte growth in particular);
3) Understanding the role of co-activator complexes in transcription regulation;
4) Investigation of of general transcription factor behavior, recruitment and RNA polymerase II transcription in living cells at transcribed genes;
5) Characterize how and why TAF mutations cause neuronal disorders and intellectual disabilities?
Laszlo Tora -
Tetrahedron Letters ; Volume: 38 ; Page: 7527-7530
Journal of Biological Chemistry ; Volume: 271 ; Page: 19774 - 19780
Nature ; Volume: 378 ; Page: 403-406