
Structural biology of epigenetic targets
Structural biology of epigenetic targets
Chromatin structure is a major barrier to all nuclear processes and its modulation is essential for correct cell growth. There is now clear evidence that defects in chromatin modulation are responsible for a wide range of diseases, including cancer. Epigenetic modifications have a large impact on chromatin structure, acting in synergy with ATP-dependent remodelers, histone variants and histone chaperones to regulate nuclear mechanisms. By combining state-of-the-art biochemical and crystallographic techniques, we aim to understand chromatin remodeling at the molecular level. Three main targets are the current focus of our research: protein arginine methyltransferases (PRMTs), histone chaperones and histone deacetylases (HDACs). Furthermore, we are also developing structurebased rational approaches to characterize small molecule modulators of the biological activities of our targets since they are frequently involved in human diseases. Our team also participates in collaborative research on several related projects which require our specific expertise. Present collaborations concern the structural studies of complexes of cancer-related targets such as the Translationally Controlled Tumor Protein (TCTP) and the mRNA of human histone H4.
Members
Researchers
Engineers
Publications
2022
Insect Cells-Baculovirus System for the Production of Difficult to Express Proteins: From Expression Screening for Soluble Constructs to Protein Quality Control
- Simon Pichard
- Nathalie Troffer-Charlier
- Isabelle Kolb-Cheynel
- Pierre Poussin-Courmontagne
- Wassim Abdulrahman
- Catherine Birck
- Vincent Cura
- A. Poterszman
Insoluble Proteins ; Volume: 2406 ; Page: 281-317
HR-Bac, a toolbox based on homologous recombination for expression, screening and production of multiprotein complexes using the baculovirus expression system
- Olga Kolesnikova
- Amélie Zachayus
- Simon Pichard
- Judit Osz
- Natacha Rochel
- Paola Rossolillo
- Isabelle Kolb-Cheynel
- Nathalie Troffer-Charlier
- Emmanuel Compe
- Olivier Bensaude
- Imre Berger
- Arnaud Poterszman
Scientific Reports ; Volume: 12 ; Page: 2030
2018
Hijacking DNA methyltransferase transition state analogues to produce chemical scaffolds for PRMT inhibitors
- Ludovic Halby
- Nils Marechal
- Dany Pechalrieu
- Vincent Cura
- Don-Marc Franchini
- Céline Faux
- Frédéric Alby
- Nathalie Troffer-Charlier
- Srikanth Kudithipudi
- Albert Jeltsch
- Wahiba Aouadi
- Etienne Decroly
- Jean-Claude Guillemot
- Patrick Page
- Clotilde Ferroud
- Luc Bonnefond
- Dominique Guianvarc'H
- Jean Cavarelli
- Paola Arimondo
Philosophical Transactions of the Royal Society B: Biological Sciences ; Volume: 373 ; Page: 20170072
2017
SECIS-binding protein 2 interacts with the SMN complex and the methylosome for selenoprotein mRNP assembly and translation
- Anne-Sophie Gribling-Burrer
- Michael Leichter
- Laurence Wurth
- Alexandra Huttin
- Florence Schlotter
- Nathalie Troffer-Charlier
- Vincent Cura
- Martine Barkats
- Jean Cavarelli
- Séverine Massenet
- Christine Allmang
Nucleic Acids Research ; Volume: 45 ; Page: 5399-5413
Structural studies of protein arginine methyltransferase 2 reveal its interactions with potential substrates and inhibitors
- Vincent Cura
- N Marechal
- N Troffer-Charlier
- Jean-Marc Strub
- M van Haren
- Nathaniel I. Martin
- Sarah Cianferani
- Luc Bonnefond
- Jean Cavarelli
FEBS J ; Volume: 284 ; Page: 77-96
Transition state mimics are valuable mechanistic probes for structural studies with the arginine methyltransferase CARM1
- Matthijs van Haren
- Nils Marechal
- Nathalie Troffer-Charlier
- Agostino Cianciulli
- Gianluca Sbardella
- Jean Cavarelli
- Nathaniel Martin
Proceedings of the National Academy of Sciences of the United States of America ; Volume: 114 ; Page: 3625-3630
Crystallization and Preliminary X-Ray Diffraction Analysis of a Mammal Inositol 1,3,4,5,6-Pentakisphosphate 2-Kinase
- Elsa Franco-Echevarria
- Julia Sanz-Aparicio
- Nathalie Troffer-Charlier
- Arnaud Poterszman
- Beatriz Gonzalez
Protein Journal ; Volume: 36 ; Page: 240-248
2015
TCTP contains a BH3-like domain, which instead of inhibiting, activates Bcl-xL.
- Stéphanie Thébault
- Morgane Agez
- Xiaoke Chi
- Johann Stojko
- Vincent Cura
- Stéphanie B Telerman
- Laurent Maillet
- Fabien Gautier
- Isabelle Billas-Massobrio
- Catherine Birck
- Nathalie Troffer-Charlier
- Teele Karafin
- Joane Honoré
- Andrea Senff-Ribeiro
- Sylvie Montessuit
- Christopher M Johnson
- Philippe Juin
- Sarah Cianférani
- Jean-Claude Martinou
- David W Andrews
- ...
Scientific Reports ; Volume: 6 ; Page: 19725
Insect Cells–Baculovirus System for the Production of Difficult to Express Proteins
- Judit Osz-Papai
- Laura Radu
- Wassim Abdulrahman
- Isabelle Kolb-Cheynel
- Nathalie Troffer-Charlier
- Catherine Birck
- A. Poterszman
Insoluble Proteins ; Volume: 1258 ; Page: 181-205
2012
Expression of functional full-length hSRC-1 in eukaryotic cells using modified vaccinia virus Ankara and baculovirus
- Judit Osz
- Karine Pradeau-Aubreton
- Robert Drillien
- Nathalie Troffer-Charlier
- Isabelle Kolb-Cheynel
- Arnaud Poterszman
- Marc Ruff
- Dino Moras
- Natacha Rochel
Analytical Biochemistry ; Volume: 426 ; Page: 106-108