Structure-fonction of nucleoproteic complexes of DNA topoisomerases
Subgroup Leader : Valerie LAMOUR
Our research group focuses on the molecular mechanisms governing nucleic acid transport, processing and topological changes of chromatin DNA. Our present targets are nucleoprotein complexes involved in retroviral DNA integration and eukaryotic DNA topoisomerases. We integrate structural data from high resolution (X-ray crystallography), medium resolution (CryoEM) and low resolution (cell imaging) with in vitro and in cellulo functional data.
The main focus of Marc Ruff is to understand the molecular processes performed by the HIV pre-integration complexes (PIC). The aim is to provide a four dimensional view of the PIC, from reverse transcription until integration. A second component of the project focuses on drug design aspects to optimize protein-protein interaction and conformational inhibitors.
Valérie Lamour’s project is aiming at the functional and structural studies of DNA topoisomerase cellular complexes targeted by therapeutic compounds used in cancer and anti-bacterial therapies. The main goal of this study is to identify and study the structure/function of Topoisomerase-associated complexes targeted by drugs through a combination of functional and structural approaches.
Subgroup Leader : Valerie LAMOUR
Subgroup Leader : Nicolas LEVY
Marlène Vayssières, postdoc
Léa Haas, engineer
Christophe Lotz, PhD student
Arnaud Vanden Broeck, PhD student
Claire Bedez, PhD student
On June 17, 2025, to mark the 20th anniversary of the Grandes Avancées Françaises en Biologie, the French Academy of Sciences welcomed researchers and…
Read more
Article in a journal
Proceedings of the National Academy of Sciences of the United States of America ; Volume: 121 ; Page: 112744
Pre-publication, Working Document
Article in a journal
BioRxiv
Article in a journal
Journal of Biological Chemistry ; Volume: 300 ; Page: 107374
Article in a journal
Science ; Volume: 384 ; Page: 227-232
Article in a journal
Bioorganic Chemistry ; Volume: 137 ; Page: 106616
Article in a journal
Antimicrobial Agents and Chemotherapy ; Volume: 67
Article in a journal
mBio ; Volume: 14 ; Page: e0108323
Article in a journal
Cell Reports ; Volume: 42 ; Page: 112744
Article in a journal » Review article
International Journal of Molecular Sciences ; Volume: 24 ; Page: 3986